Bromophenol Blue: A Versatile pH Indicator and Electrophoresis Tracking Dye
Unlock precise pH measurements and visualize molecular migration with this essential laboratory reagent.
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Bromophenol Blue
Bromophenol Blue is a highly versatile chemical compound primarily utilized as a pH indicator and a tracking dye in electrophoresis. Its reliable color transitions offer clear visual cues for accurate pH monitoring and experimental progress tracking.
- As a pH indicator, Bromophenol Blue provides a clear visual transition from yellow in acidic conditions to blue in alkaline environments, making pH determination straightforward.
- The compound's role as an electrophoresis tracking dye allows researchers to easily visualize the migration of biomolecules like DNA, RNA, or proteins during gel electrophoresis.
- Understanding the specific bromophenol blue pH indicator range (pH 3.0-4.6) is crucial for its accurate application in various titration and biochemical assays.
- The chemical properties of bromophenol blue, including its solubility and stability, contribute to its widespread adoption in scientific research settings.
Key Advantages
Precise pH Indication
Leverage the sharp color changes of bromophenol blue for accurate pH measurements, essential for many laboratory procedures.
Visible Electrophoresis Tracking
Easily monitor sample movement in gel electrophoresis with this bright, distinct tracking dye.
Versatile Application
From titrations to biochemical studies, bromophenol blue serves multiple roles in scientific research, making it a cost-effective choice.
Key Applications
pH Measurement
Utilize bromophenol blue's distinct color shift to accurately determine the pH of various solutions in scientific research.
Gel Electrophoresis
Employ bromophenol blue as a tracking dye to visualize and monitor the progress of DNA, RNA, and protein separation in gels.
Titration
Effectively signal the endpoint in acid-base titrations, ensuring precise experimental outcomes.
Biochemical Studies
Support research in enzyme kinetics and protein analysis through its visual indicator properties.