4-Chloro-7-Nitrobenzofurazan: A Versatile Fluorescent Labeling Reagent for Biomolecule Analysis
Unlocking fluorescence in biomolecular research and analytical chemistry with a key organic intermediate.
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4-Chloro-7-nitrobenzofurazan
This compound serves as a pivotal organic intermediate, renowned for its utility as a fluorescent molecule in various scientific disciplines. Its ability to react with amines and thiols to generate fluorescent adducts makes it indispensable for precise molecular tagging and analysis.
- Discover the power of 4-chloro-7-nitrobenzofurazan for peptide labeling, enabling detailed studies of peptide structures and interactions.
- Leverage NBD-Cl as a chromatography reagent for enhanced separation and detection of amino acids in complex biological samples.
- Achieve superior fluorescent labeling of proteins, crucial for visualizing and quantifying protein expression and localization.
- Explore the synthesis of other heterocyclic molecules using this versatile organic intermediate.
Key Advantages Provided
Enhanced Biomolecular Detection
The reactivity of NBD-Cl with amines facilitates the creation of highly fluorescent compounds, significantly boosting the sensitivity in biomolecule analysis and assays.
Chromatographic Precision
As a specialized chromatography reagent, it improves the separation and detection of amino acids and other low molecular weight amines in HPLC analysis.
Synthetic Versatility
This compound acts as a fundamental organic intermediate, essential for the synthesis of diverse heterocyclic structures and fluorescent derivatives.
Key Applications
Biochemistry
Essential for the fluorescent labeling of peptides, proteins, and other biomolecules, aiding in research and diagnostics.
Chromatography
Utilized as a derivatizing reagent for HPLC analysis, particularly for amino acids and low molecular weight amines, enabling precise quantitative measurements.
Organic Synthesis
Serves as a key intermediate in the creation of novel heterocyclic compounds and functionalized fluorescent labels.
Analytical Chemistry
Functions as a sensitive chromogenic and fluorogenic reagent for the detection and quantification of various analytes.