5-Bromo-4-chloro-3-indolyl Phosphate Disodium Salt: A High-Purity Substrate for Alkaline Phosphatase Detection
Unlock precise biological detection with our premium BCIP, essential for alkaline phosphatase assays.
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5-Bromo-4-chloro-3-indolyl Phosphate Disodium Salt
This high-purity compound serves as a critical chromogenic substrate for the sensitive detection of alkaline phosphatase (AP) activity. It plays a vital role in various research and diagnostic applications by producing a visible color change upon enzymatic reaction, enabling precise localization and quantification.
- Enables accurate colorimetric detection of alkaline phosphatase activity, a key marker in numerous biological studies.
- Versatile use in applications like Western blotting and immunohistochemistry, contributing to reliable signal detection.
- Facilitates precise in situ hybridization by providing a clear visual indicator of enzyme presence.
- Serves as a crucial component in the BCIP/NBT substrate system, enhancing detection sensitivity for various assays.
Advantages Offered by the Product
High Sensitivity and Specificity
Experience superior detection capabilities with this substrate, ensuring specific identification of alkaline phosphatase activity, crucial for detailed molecular biology studies.
Versatile Application Range
From immunoblotting to diagnostics, its broad applicability makes it a go-to reagent for researchers and technicians needing reliable enzyme detection.
Enhanced Visualization
The formation of a distinct colored precipitate upon enzymatic hydrolysis allows for clear visualization, simplifying data interpretation in complex biological assays.
Key Applications
Histochemistry
Utilized as a histochemical substrate for alkaline phosphatase, aiding in the visualization of enzyme activity in tissue samples for research.
Molecular Biology
Essential for techniques like Northern, Southern, and Western blotting, offering reliable detection of target molecules.
Immunohistochemistry
A key component in immunohistochemistry for detecting AP-labeled antibodies and visualizing cellular localization of enzymes.
In Situ Hybridization
Used in in situ hybridization to provide a chromogenic signal for the presence of specific nucleic acid sequences.