Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate): A Versatile Chromogenic Substrate for Peroxidase Assays and ELISA
Unlock sensitive enzymatic detection with our high-purity diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate), perfect for your ELISA and biochemical analysis needs.
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Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
This essential compound serves as a highly sensitive chromogenic substrate for peroxidase enzyme detection, widely adopted in enzyme-linked immunosorbent assays (ELISA). Its ability to produce a soluble green product upon reaction with hydrogen peroxide and peroxidase allows for accurate spectrophotometric analysis, typically at 405 nm. The reaction's endpoint can be effectively managed using a 1% SDS solution, ensuring reliable and reproducible results in various biochemical applications.
- Reliable ELISA Detection: Facilitates accurate enzyme-linked immunosorbent assays by acting as a sensitive chromogenic substrate for peroxidase.
- Efficient Enzyme Reaction Monitoring: Essential for observing and quantifying enzyme reaction kinetics, crucial for understanding biological processes.
- Food Industry Application: Plays a vital role in measuring the antioxidant capacities of foods, contributing to food quality and safety assessments.
- Glucose Estimation: Utilized as a component in glucose estimating reagents for blood serum analysis, aiding in diagnostic procedures.
Key Advantages of Using This Substrate
High Sensitivity
Leverage the high sensitivity of this diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) for precise detection in your enzyme assays, ensuring that even low concentrations are accurately identified.
Versatile Application
This compound is a versatile reagent, finding application not only in ELISA but also in broader biochemical analysis and as a key component in glucose estimating reagents for blood serum.
Clear Product Formation
The formation of a distinct green, water-soluble product allows for straightforward spectrophotometric measurement, simplifying data interpretation and analysis.
Key Applications
Enzyme-Linked Immunosorbent Assays (ELISA)
The substrate is widely utilized as a sensitive chromogenic substrate for peroxidase in ELISA procedures, enabling accurate antibody and antigen detection.
Antioxidant Capacity Measurement
It plays an important role in the food industry, facilitating the measurement of antioxidant capacities of various food products.
Enzyme Reaction Kinetics
Used to observe and study the reaction kinetics of specific enzymes, providing insights into enzyme mechanisms and activity.
Clinical Diagnostics
Involved in glucose estimating reagents for blood serum, supporting clinical diagnostic tests and health monitoring.