Fluorexon (CAS 1461-15-0): A Versatile Fluorescent Dye for Metal Ion Analysis and Cell Viability Assays
Explore the critical role of Fluorexon in precise metal ion determination and advanced cellular research.
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Fluorexon
Fluorexon, identified by CAS number 1461-15-0, is a sophisticated fluorescent dye that serves a dual purpose in scientific applications. Its primary function involves precise metal ion determination, acting as a crucial reagent for quantifying elements such as calcium, strontium, copper, and manganese. Furthermore, it functions effectively as a complexometric indicator in EDTA titrations, enabling accurate analysis in the presence of challenging matrices like magnesium. The derivative, Calcein AM, extends its utility into cell biology, facilitating the assessment of cell viability and enabling short-term cell labeling due to its ability to penetrate cell membranes and emit a bright green fluorescence within living cells.
- Leveraging Fluorexon for precise calcium determination in various biological and chemical samples through advanced fluorescence spectroscopy.
- Discover the advantages of using Calcein AM in cell labeling for tracking cellular processes and identifying live cells in complex biological systems.
- Understand the principles of EDTA titration using Calcein as an indicator for accurate quantification of metal ions, even in the presence of interfering substances.
- Learn about the critical steps in Fluorexon preparation and purification to ensure high assay sensitivity and reliable results for chemical analysis.
Key Advantages
High Sensitivity in Metal Ion Detection
Utilize Fluorexon's fluorescent properties to achieve highly sensitive detection of metal ions, crucial for accurate chemical analysis.
Reliable Indicator for Titration
Employ Fluorexon as a dependable indicator for EDTA titrations, ensuring reproducible results in quantitative chemical analysis.
Versatile Cell Biology Applications
Benefit from the dual functionality of Fluorexon and its derivative, Calcein AM, for comprehensive cell viability testing and advanced cellular research.
Key Applications
Metal Ion Quantification
Accurately determine the concentration of various metal ions, including calcium, strontium, copper, and manganese, utilizing the fluorescent properties of Fluorexon for sensitive measurements.
Complexometric Titrations
Serve as a key indicator in EDTA titrations, facilitating the precise quantification of metal ions, particularly calcium, even in the presence of magnesium.
Cell Viability Assays
Employ Calcein AM, a membrane-permeable derivative, to assess cellular health and identify live cells, a critical aspect in biological and pharmaceutical research.
Cellular Labeling and Tracking
Utilize Calcein AM for short-term labeling and tracking of cells, offering insights into cellular processes, migration, and interactions in various biological studies.