High Purity Tetramethylbenzidine (TMB): A Safe and Sensitive Chromogenic Substrate
Discover the advantages of TMB for your enzyme immunoassays and diagnostic applications.
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Tetramethylbenzidine (TMB)
Tetramethylbenzidine (TMB) is a cutting-edge chromogenic substrate extensively utilized in enzyme immunoassay (EIA) and ELISA. It serves as a safer, non-carcinogenic alternative to traditional benzidine-based compounds, offering enhanced sensitivity and reliable signal generation in various diagnostic and research contexts. Its ability to participate in Horseradish Peroxidase (HRP) catalyzed reactions makes it an indispensable tool for biochemical analysis.
- Leverage the benefits of a safe chromogenic reagent for your HRP catalyzed reactions, ensuring accuracy in diagnostics.
- Utilize Tetramethylbenzidine CAS 54827-17-7 as a key component in your Enzyme Immunoassay protocols for precise results.
- Explore the high sensitivity offered by TMB substrate for ELISA, improving the detection limits of your assays.
- Benefit from Tetramethylbenzidine as a non-carcinogenic benzidine substitute, prioritizing safety in laboratory practices.
Key Advantages
Safety and Sensitivity
TMB offers a superior safety profile compared to older substrates, acting as a non-carcinogenic benzidine substitute while maintaining high sensitivity for enzyme immunoassays.
Versatile Application
As a crucial TMB substrate for ELISA and EIA, it is fundamental for numerous diagnostic tests, including occult blood detection and viral hepatitis screening.
Robust Reaction Mechanism
The TMB oxidation mechanism, driven by HRP and hydrogen peroxide, produces distinct color changes enabling quantitative analysis, crucial for accurate biochemical analysis.
Key Applications
Clinical Diagnostics
Essential for clinical biochemical tests, TMB serves as a peroxidase substrate in EIA and ELISA for detecting analytes like blood and viral markers.
Forensic Science
Its sensitivity makes it valuable for forensic examinations, such as fingerprint detection and alcohol level determination in saliva.
Research and Development
Widely adopted in R&D for developing new diagnostic assays and understanding enzyme kinetics, benefiting from its predictable TMB oxidation mechanism.
Environmental Monitoring
Used in applications requiring sensitive detection of substances in environmental samples, leveraging its properties as a safe chromogenic reagent.