Nitrotetrazolium Blue Chloride: A Vital Enzyme Activity Detection Reagent
Unlock precise enzyme activity insights with high-purity Nitrotetrazolium Blue Chloride, essential for research.
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Nitrotetrazolium Blue Chloride
Nitrotetrazolium blue chloride is a crucial reagent for accurately detecting the activity of dehydrogenases and oxidases. Its ability to catalyze the formation of insoluble blue products makes it indispensable in various biochemical and cellular analysis techniques.
- This reagent is critical for assays designed to detect dehydrogenase activity, offering reliable results in biological research.
- As a substrate for dehydrogenases and other peroxidases, it's vital for studies focusing on enzyme kinetics and cellular metabolic pathways.
- The use of Nitrotetrazolium Blue Chloride in combination with BCIP allows for sensitive detection of superalkaline phosphatase activity, a key biomarker in many biological processes.
- Researchers frequently utilize this product for the determination of glucose-6-phosphate dehydrogenase levels, contributing to diagnostic and research applications.
Key Advantages Provided
High Purity and Reliability
Ensuring consistent and accurate results, the high purity of this enzyme substrate is paramount for successful experimental outcomes.
Versatile Detection Capabilities
Effectively employed in IHC and ICC, this product allows for the visualization of enzyme activity directly within cellular structures.
Essential for Blotting Techniques
Its application in Western Blot assays aids in the detection of specific proteins by visualizing their enzymatic activity or associated markers.
Key Applications
Enzyme Activity Assays
Utilized as a primary enzyme substrate, this compound facilitates the quantitative and qualitative analysis of various enzyme activities in biological samples.
Immunohistochemistry (IHC)
Essential for IHC, it helps visualize enzyme localization and activity within tissue sections, crucial for disease research.
Western Blotting (WB)
In Western Blot, it acts as a chromogenic substrate, enabling the detection of specific protein bands through visualized enzymatic reactions.
Biochemical Research
Researchers leverage this product for studies involving glucose-6-phosphate dehydrogenase and blood coagulation factor I precipitation, advancing scientific understanding.
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