Phenazine Methosulfate CAS 299-11-6: An Essential Electron Carrier for Biochemical Assays and Research
Explore the critical role of Phenazine Methosulfate as a vital redox dye in advancing scientific research and diagnostics.
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Phenazine Methosulfate
Phenazine Methosulfate (PMS) with CAS number 299-11-6 is a powerful electron carrier, indispensable for a range of biochemical applications. Its ability to facilitate electron transfer between biological molecules and detection systems makes it a cornerstone in many laboratory protocols.
- As a key electron carrier, Phenazine Methosulfate is vital for coupling the production of NADH or NADPH to the reduction of tetrazolium salts, a process central to many biochemical assays.
- It plays a crucial role in the determination of nitric oxide reductase activity, making it an important reagent for understanding various metabolic pathways.
- Researchers widely utilize Phenazine Methosulfate in cell viability assays, acting as an electron transfer reactant to provide reliable indicators of cellular health and response.
- Furthermore, its application extends to the preparation of succinic dehydrogenase, highlighting its versatility in enzyme-related research.
Key Advantages
Broad Applicability
The broad applicability of Phenazine Methosulfate spans various scientific disciplines, from enzymology to cell biology, making it a versatile tool for researchers exploring redox properties.
Enzyme Functionality Enhancement
By acting as an artificial electron acceptor and carrier, Phenazine Methosulfate enhances the functionality of many enzymes in research settings, contributing to more accurate and sensitive assay results.
Reliable Research Indicator
Its well-defined role in electron transfer processes makes Phenazine Methosulfate a reliable indicator for studying enzyme kinetics and mechanisms of action in complex biological systems.
Key Applications
Biochemical Assays
Phenazine Methosulfate is a cornerstone in numerous biochemical assays, facilitating precise measurements of enzyme activity and metabolic processes.
Electron Transfer Studies
Its function as an electron carrier makes it invaluable for studies investigating electron transfer pathways in biological systems, crucial for understanding cellular respiration and energy production.
Enzyme Kinetics
Researchers leverage Phenazine Methosulfate to explore enzyme kinetics, understanding reaction rates and mechanisms, which is fundamental for drug discovery and development.
Cell Viability Testing
In cell viability testing, Phenazine Methosulfate provides a reliable method to assess the metabolic activity and health of cells under various experimental conditions.