PIPES: The Ultimate Biological Buffer for Precise pH Control in Scientific Research
Unlock unparalleled pH stability for your critical biochemical and cell biology experiments.
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PIPES Biological Buffer
PIPES, identified by CAS number 5625-37-6, stands as a vital zwitterionic biological buffer, indispensable for maintaining stable pH levels in a wide array of scientific applications. Its chemical structure, C8H18N2O6S2, allows it to function effectively within a pH range of 6.1-7.5, making it particularly suitable for biological systems that operate near physiological conditions. Researchers frequently rely on this buffer for its ability to minimize lipid loss during glutaraldehyde fixation in histological preparations of both plant and animal tissues.
- Leverage PIPES buffer for protein purification, ensuring optimal conditions for isolating and characterizing your target proteins.
- Achieve superior results in chromatography by utilizing the stable pH environment provided by PIPES buffer in chromatography applications.
- Explore the critical PIPES buffer uses in research, from foundational biochemistry to cutting-edge cell biology studies.
- Ensure the health and viability of your cell cultures with reliable pH stabilization from PIPES buffer for cell culture.
Key Advantages Delivered
Exceptional pH Stability
Benefit from the precise buffering capabilities of PIPES, offering a consistent PIPES buffer pKa range of 6.1 – 7.5, ideal for sensitive biological processes.
Minimized Metal Ion Interaction
Its weak interaction with most metal ions makes PIPES an excellent choice for experiments where metal ion chelation could interfere with results, a key aspect of its PIPES buffer chemical properties.
Versatile Application Range
From protein purification to cell culture, PIPES demonstrates its versatility, solidifying its role in diverse research fields and as a trusted PIPES buffer for protein purification.
Key Applications
Cell Culture Media
Maintain optimal pH for cell growth and viability, a critical factor supported by reliable PIPES buffer for cell culture applications.
Protein Purification
Ensure the integrity and functionality of proteins during purification processes using the stable buffering environment of PIPES, a common use in PIPES buffer uses in research.
Chromatography
Achieve better separation and analysis in chromatographic techniques by employing PIPES for its consistent pH control.
Biochemical Assays
Support a wide range of biochemical reactions and assays with the dependable buffering capacity provided by PIPES.