Understanding BES: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic Acid as a Superior Biological Buffer
Discover the key properties and versatile applications of BES buffer in modern biological research and diagnostics.
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N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid
BES is a zwitterionic buffer known for its excellent buffering capacity in the physiological pH range, making it invaluable for a wide array of biochemical and molecular biology applications. Its chemical stability and low UV absorbance contribute to its reliability in sensitive experimental setups.
- The precise pKa of 7.15 at 20°C makes it ideal for maintaining stable pH in biological assays, preventing unwanted fluctuations that could affect experimental outcomes.
- High water solubility ensures ease of use and consistent performance in various buffer systems, a crucial aspect for reproducible research.
- Its utility in calcium phosphate-DNA complex formation is key for effective cell transfection protocols, a fundamental technique in genetic research.
- BES buffer can also play a role in protein assays, where it minimizes interference with colorimetric measurements, thus enhancing assay accuracy.
Advantages Offered by the Product
Exceptional pH Stability
Leveraging its zwitterionic nature, BES provides robust buffering capacity, crucial for experiments requiring consistent pH levels. This characteristic is vital when working with sensitive biological systems.
Versatile Application in Research
From facilitating calcium phosphate DNA transfection to acting as a reliable component in diagnostic assay manufacturing, BES offers broad utility across different scientific disciplines.
Enhanced Assay Accuracy
Studies show that BES can diminish interference in protein assays, leading to more accurate quantitative results. This makes it a preferred choice for biochemistry and molecular biology research where precision is paramount.
Key Applications
Biological Research
BES serves as a critical buffer in numerous biological assays, ensuring optimal conditions for enzymatic reactions and cellular processes.
Cell Culture
Its stability and compatibility with cell systems make it a valuable component in cell culture media, supporting cell growth and viability.
Diagnostic Assays
The manufacturing of diagnostic assays often relies on buffers like BES to maintain the integrity and performance of reagents.
Gene Transfection
As a key component in calcium phosphate precipitation, BES significantly aids in the efficient transfection of eukaryotic cells with DNA.
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