Understanding Fmoc-ACA-OH: A Key Fluorescent Labeling Reagent in Peptide Synthesis
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Fmoc-ACA-OH
Fmoc-ACA-OH is a specialized chemical compound designed for advanced applications in peptide synthesis. Its primary utility lies in its capability to introduce a fluorescent label onto peptide chains, a critical step for researchers investigating enzyme activity and molecular interactions. The unique spectral properties conferred by Fmoc-ACA-OH, including an excitation wavelength maximum of 325 nm and an emission maximum of 400 nm, make it invaluable for FRET studies and other sensitive biochemical assays. This compound plays a vital role in preparing peptides with a fluorescent label at the C-terminal, offering enhanced detection capabilities and analytical precision in life science research.
- Enabling precise peptide synthesis with fluorescent labels is crucial for downstream applications in biochemical research.
- The compound's spectral characteristics, featuring an excitation wavelength maximum of 325 nm, allow for detailed fluorescence microscopy and spectroscopy.
- Its specific emission maximum of 400 nm ensures clear signal detection, vital for accurate quantitative analysis in enzyme studies.
- Fmoc-ACA-OH is instrumental in the effort to prepare peptides with fluorescent labels, expanding their utility in various biological contexts.
Key Advantages Provided
Enhanced Detection Capabilities
The integration of Fmoc-ACA-OH allows for the development of peptides with superior fluorescent properties, directly improving detection sensitivity in complex biological samples.
Facilitates Enzyme Studies
By enabling the preparation of peptides with specific spectral signatures, this reagent is key for researchers performing enzyme kinetic studies and developing novel peptide substrates.
High Purity and Reliability
With a purity exceeding 98%, Fmoc-ACA-OH ensures consistent and reliable results in demanding peptide synthesis protocols, minimizing experimental variability.
Key Applications
Peptide Synthesis
Fmoc-ACA-OH is a critical building block in solid-phase peptide synthesis (SPPS), utilized for its unique chemical structure and reactivity in creating custom peptide sequences.
Fluorescent Labeling
Its primary application is in fluorescent labeling, enabling the creation of peptides with specific spectral characteristics for advanced biological research and diagnostics.
Biochemical Assays
Researchers leverage Fmoc-ACA-OH to prepare fluorescently tagged peptides that serve as sensitive substrates for enzyme activity assays and protein interaction studies.
Life Science Research
This reagent supports a broad range of life science investigations, from molecular biology to drug discovery, by providing tools for precise peptide modification and analysis.