DTE: A Powerful Reducing Agent for Biological Applications
Discover the essential role of Dithioerythritol in protein science, enzyme stability, and biochemical research.
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Dithioerythritol (DTE)
DTE, or Dithioerythritol, is a critical biochemical reagent widely recognized for its potent reducing capabilities. Appearing as a white to off-white solid powder with a melting point of 42-43°C, DTE plays a vital role in maintaining the integrity and functionality of proteins and enzymes.
- Prevent protein oxidation DTE: DTE actively prevents the oxidation of free sulfhydryl residues, safeguarding protein structures from degradation.
- Break disulfide bonds with DTE: Its primary function is to efficiently break disulfide bonds within proteins, a crucial step in many biochemical analyses.
- DTE for SDS-PAGE: DTE is extensively used in SDS-PAGE to ensure complete reduction of disulfide bonds, leading to more accurate protein separation.
- Biochemical reducing agents DTE: As a superior alternative to other reducing agents, DTE ensures consistent and reliable results in sensitive biochemical assays.
Key Advantages of Using DTE
Enhanced Protein Stability
DTE acts as an exceptional enzyme stabilizer, preserving the activity of proteins during purification and experimental procedures, a benefit highlighted in DTE protein stabilization research.
Reliable Disulfide Bond Reduction
The effectiveness of DTE in breaking disulfide bonds makes it indispensable for studies involving protein structure and function, providing reliable outcomes in complex biological systems.
Prevention of Aggregation
By preventing the oxidation of cysteine residues, DTE significantly reduces the likelihood of non-specific aggregation, ensuring sample homogeneity and preserving protein activity.
Key Applications of DTE
Protein Purification & Analysis
DTE is crucial for maintaining protein solubility and preventing disulfide bond formation during purification, ensuring the recovery of active protein targets.
Electrophoresis (SDS-PAGE)
In SDS-PAGE, DTE guarantees the complete reduction of disulfide bonds, essential for accurate molecular weight determination and protein analysis.
Enzymatic Reactions
DTE serves as an enzyme stabilizer, supporting the optimal performance of enzymes in various biochemical reactions and assays.
Thiolated DNA Manipulation
It is used to protect thiolated DNA from dimerization and to ensure efficient coupling reactions in biosensor development and molecular biology applications.