AMPPD: A High-Sensitivity Chemiluminescent Substrate for Alkaline Phosphatase in Immunoassays
Unlock precise and sensitive detection with AMPPD, the advanced chemiluminescent substrate for alkaline phosphatase.
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AMPPD
AMPPD serves as a critical chemiluminescent substrate for alkaline phosphatase, pivotal in generating high-intensity light signals upon enzymatic catalysis. This controlled decomposition yields AMP-D, with the luminescence speed directly correlating to the alkaline phosphatase concentration, making it an invaluable tool for detecting hybridized molecules when alkaline phosphatase is conjugated to a probe.
- Discover the principle behind chemiluminescent assay detection with AMPPD. This substrate is designed to produce a strong light output when acted upon by alkaline phosphatase.
- Achieve sensitive detection of hybridized molecules by utilizing the direct correlation between alkaline phosphatase concentration and the generated light signal.
- Explore the use of AMPPD CAS 122341-56-4 in advanced biological assays, offering a reliable method for molecular detection.
- Understand the benefits of using a high intensity light signal substrate for improved assay performance and reduced detection limits.
Key Advantages
High Sensitivity Detection
Leverage AMPPD's ability to generate intense light signals, enabling the detection of low-abundance targets and enhancing the overall sensitivity of your assays.
Precise Quantification
The luminescence speed directly corresponds to alkaline phosphatase concentration, allowing for accurate quantitative analysis in various biological applications.
Versatile Application
AMPPD is a cornerstone for numerous chemiluminescent immunoassay strategies, particularly in research where detecting hybridized molecules is crucial.
Key Applications
Chemiluminescent Immunoassays (CLIA)
AMPPD is essential for CLIA, facilitating the detection of antigens, antibodies, and nucleic acid probes through enzyme-catalyzed light emission.
Enzyme-Linked Assays
It functions effectively in enzyme-linked assays where alkaline phosphatase activity needs to be translated into a measurable light signal for research purposes.
Molecular Hybridization Detection
AMPPD provides a highly sensitive method for detecting hybridized molecules by linking the reporter enzyme (alkaline phosphatase) to the probe.
Biochemical Research
Its predictable light-emitting properties make it a valuable reagent in general biochemical research for tracking enzyme activity and molecular interactions.