In the intricate field of histology, precise and reliable staining techniques are fundamental for visualizing cellular structures and understanding tissue morphology. Among the array of stains employed, Acid Orange 10 (CAS 1936-15-8), also known as Orange G, stands out for its specific utility in differentiating cellular components. This article aims to inform laboratory managers, researchers, and procurement specialists about the significance of Acid Orange 10 in histological applications and guide them on sourcing this essential chemical.

Acid Orange 10 is an azo dye that has found widespread use as a histological stain. Its vibrant orange color and affinity for certain cellular elements make it an invaluable tool in various staining protocols. It is frequently used in combination with other stains, such as hematoxylin and eosin (H&E) or in more complex multi-stain techniques like Masson's trichrome or Kornhauser's quadruple stain. These combinations allow for the clear demarcation of cytoplasm, muscle fibers, collagen, and other connective tissues.

The primary function of Acid Orange 10 in histology is often to stain cytoplasm, keratin, and muscle fibers, appearing as an orange to reddish-orange hue. Its effectiveness lies in its differential staining capabilities. For instance, in the context of detecting lung cancer metastasis, its interaction with specific cellular components aids researchers. It's also utilized in studies measuring glycated proteins and in ophthalmic device applications, highlighting its diverse biological relevance.

For laboratories and research institutions, ensuring the quality and consistency of histological stains is paramount. When purchasing Acid Orange 10, it is crucial to source it from reputable manufacturers and suppliers who guarantee high purity and certification for biological staining. Information regarding its solubility (e.g., readily soluble in water), pH characteristics, and extinction coefficient (λmax) can be beneficial for optimizing staining protocols. Buyers looking to purchase this dye should verify that the product meets the specific requirements for their intended use, whether it's routine histology or advanced research.

Reliable suppliers typically offer Acid Orange 10 in various quantities, catering to different laboratory needs, from small research batches to bulk supplies for large institutions. Procurement managers should seek out suppliers who provide clear technical specifications, safety data sheets (SDS), and prompt customer service. The price of Acid Orange 10 can vary based on purity, quantity, and supplier, so obtaining quotes from multiple trusted sources is advisable. Manufacturers in China are often a good source for cost-effective, high-quality Acid Orange 10.

Furthermore, understanding the stability and handling requirements of Acid Orange 10 is important. While stable under recommended storage conditions (dry, cool, well-ventilated, sealed, and shaded), it's important to be aware of potential incompatibilities with strong oxidizing agents. Adhering to safety guidelines provided by the supplier ensures safe handling and storage in the laboratory environment.

In summary, Acid Orange 10 is a cornerstone stain in many histological laboratories. Its distinct staining properties and compatibility with various multi-staining techniques make it indispensable for accurate tissue analysis. By prioritizing quality suppliers and understanding the dye's characteristics, researchers can confidently procure Acid Orange 10 for their critical work in understanding cellular and tissue structures.