In the fast-paced world of research and development (R&D), the right tools can make all the difference. Proteinase K stands out as an exceptionally versatile enzyme, finding its place in a multitude of critical applications. As a dedicated manufacturer and supplier based in China, we are committed to providing R&D professionals with high-quality Proteinase K that drives innovation and ensures experimental success.

Proteinase K, with its CAS number 39450-01-6, is a robust serine protease renowned for its broad substrate specificity. This means it can effectively digest a wide range of proteins, making it invaluable for numerous laboratory procedures. Whether you are working in molecular biology, diagnostics, or upstream bioprocessing, understanding the applications of Proteinase K can significantly enhance your R&D efforts.

One of the most prominent uses of Proteinase K is in the preparation of nucleic acids. During DNA and RNA extraction, the presence of endogenous nucleases can be a significant challenge, leading to the degradation of valuable genetic material. By incorporating Proteinase K into the lysis buffer, researchers can effectively inactivate these nucleases, ensuring the integrity and purity of extracted DNA and RNA. This is fundamental for downstream applications such as PCR, gene sequencing, and microarray analysis. Our role as a manufacturer is to supply an enzyme that reliably performs this critical function.

Beyond nucleic acid purification, Proteinase K is also utilized in protein fingerprinting and for the removal of contaminating proteins in various biochemical preparations. Its ability to digest proteins that are often resistant to denaturation, coupled with its stability in the presence of common detergents and denaturants, makes it a preferred choice in challenging protocols. For instance, in the isolation of intact, high-molecular-weight DNA, such as genomic DNA for PFGE (Pulsed-Field Gel Electrophoresis), Proteinase K plays a crucial role in disrupting protein-DNA complexes and inactivating DNases.

Furthermore, Proteinase K is employed in diagnostics for sample preparation, where rapid and efficient degradation of host cellular material is required. In the biopharmaceutical industry, it can be used in upstream processing to remove host cell proteins, thereby improving the purity of therapeutic proteins. As a China-based supplier, we cater to these diverse R&D needs, offering Proteinase K with a consistent assay of ≧95% and activity of 30.0-45.0 u/mg.

For R&D teams looking to optimize their workflows and secure a reliable supply, buying Proteinase K directly from a manufacturer like us offers significant advantages. We provide not only competitive pricing but also the assurance of quality and lot-to-lot consistency. We encourage you to explore how our high-purity Proteinase K can benefit your specific research and development projects. Contact us today to discuss your requirements and to obtain a quote for this essential biochemical reagent.