Understanding the underlying chemistry of reagents is crucial for optimizing experimental outcomes. NINGBO INNO PHARMCHEM CO.,LTD. offers 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (BCIP), a compound whose chemical structure and properties make it an invaluable tool in enzyme-activated detection systems. As a leading biochemical supplier, we provide insights into how this reagent functions and why its quality is paramount for accurate scientific results.

BCIP is a synthetic substrate that undergoes hydrolysis by alkaline phosphatase. This enzymatic reaction cleaves the phosphate group, releasing 5-bromo-4-chloro-3-indoxyl. This intermediate is unstable and rapidly oxidizes, dimerizing to form 5,5'-dibromo-4,4'-dichloro-indigo, a richly colored blue precipitate. When used in combination with Nitro Blue Tetrazolium (NBT), the resulting precipitate is a deep purple, offering excellent contrast for visual identification. The chemical reaction mechanism is fundamental to its use in molecular biology research and diagnostic applications. Researchers often search for the optimal price for BCIP to integrate into their workflows.

The stability and solubility characteristics of BCIP are also important considerations. Typically supplied as a white to light yellow powder, it needs to be stored properly, often at low temperatures and protected from light, to maintain its integrity. NINGBO INNO PHARMCHEM CO.,LTD., as a dedicated manufacturer in China, ensures that our BCIP meets high purity standards, which directly impacts the efficiency and reliability of the enzymatic reaction. This consistency is vital for reproducible outcomes in gene expression studies and protein detection.

The application of BCIP in enzyme-activated detection systems is broad. In cell biology, it can help researchers track the activity of specific enzymes involved in signaling pathways. In drug development, it can be used to screen for compounds that modulate enzyme activity. The ability to buy BCIP from a trusted supplier like NINGBO INNO PHARMCHEM CO.,LTD. means you are accessing a reagent that has undergone rigorous quality control, ensuring its chemical integrity. This makes it a dependable choice for demanding research environments.

For any laboratory performing assays that rely on alkaline phosphatase detection, BCIP is an essential component. NINGBO INNO PHARMCHEM CO.,LTD. is committed to providing researchers with access to high-quality biochemicals like BCIP for alkaline phosphatase detection. Our role as a supplier in China allows us to offer competitive pricing, supporting scientific advancement globally. Explore the chemical elegance of BCIP and partner with NINGBO INNO PHARMCHEM CO.,LTD. for your critical research needs.