In the sophisticated landscape of life sciences research, the development of highly specific and sensitive detection probes is paramount. Biotinylation, a versatile chemical modification, plays a crucial role, but achieving optimal signal amplification often requires more than just standard biotin conjugates. Biotin-XX Tyramide, also known as Biotin-Ahx-Ahx-Tyramide (CAS 851113-28-5), represents a significant evolution in biotinylation technology, offering enhanced performance for a variety of applications.

Understanding Biotin-XX Tyramide

Biotin-XX Tyramide is a specialized biotinylation reagent designed for use in conjunction with enzymatic amplification systems, most notably the Tyramide Signal Amplification (TSA) method. Its defining characteristic is the inclusion of an extended linker arm, consisting of two aminohexanoic acid (Ahx) units, connecting the biotin moiety to the tyramine functional group. This longer linker arm is not merely a structural addition; it is engineered to optimize the interaction with enzymes like Horseradish Peroxidase (HRP), facilitating a more efficient deposition of biotin molecules. This leads to a substantially amplified detection signal compared to shorter-chain biotin-tyramide conjugates.

Key Advantages and Applications

The enhanced performance of Biotin-XX Tyramide translates into tangible benefits for researchers:

  • Increased Sensitivity: The amplified signal allows for the detection of targets present at very low concentrations, which might be missed with less efficient reagents.
  • Improved Specificity: By facilitating a more localized deposition of biotin, TSA with Biotin-XX Tyramide can lead to sharper localization and reduced background noise.
  • Versatility: It is highly effective in various applications, including:
    • Immunohistochemistry (IHC): For superior visualization of protein expression in tissue sections.
    • Immunocytochemistry (ICC): For precise protein localization in cell cultures.
    • Western Blotting: To enhance the detection of low-abundance proteins.
    • In Situ Hybridization (ISH): For improved nucleic acid detection.

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To advance your detection capabilities and achieve more sensitive results in your biological assays, consider incorporating Biotin-XX Tyramide into your protocols. We invite you to contact us to learn more about this product, discuss your specific needs, and secure a reliable supply of this essential biotinylation tool.