Fluorescent Probes for Studying Protein Function
Understanding the intricate functions of proteins within living cells is a cornerstone of modern biology. Fluorescent probes, created by labeling proteins with bright and stable fluorophores, have revolutionized our ability to visualize and track these essential molecules. Among these versatile tools, 5(6)-Carboxyfluorescein N-succinimidyl Ester stands out for its efficacy in generating high-quality biological fluorescent derivatives. As a leading manufacturer in China, NINGBO INNO PHARMCHEM CO.,LTD. provides researchers with this critical reagent.
The succinimidyl ester functionality of 5(6)-Carboxyfluorescein N-succinimidyl Ester makes it highly reactive towards primary amine groups, which are abundant in proteins, particularly on lysine residues and N-termini. This facile reaction allows for the efficient covalent attachment of the fluorescein moiety to the protein of interest. The resulting fluorescent protein conjugates can then be utilized in a variety of biochemical and cell biology applications. For example, researchers can prepare fluorescent derivatives of proteins like tubulin, enabling detailed studies of cytoskeletal dynamics using techniques such as fluorescence microscopy and flow cytometry.
The application of these biological fluorescent derivatives extends to investigating protein localization within different cellular compartments, tracking protein-protein interactions, and monitoring protein trafficking. The bright fluorescence of the fluorescein tag allows for sensitive detection, even at low protein concentrations. This is particularly important when studying rare proteins or transient cellular events. Researchers looking to buy fluorescent labeling agents can rely on the consistent quality offered by NINGBO INNO PHARMCHEM CO.,LTD. for these demanding experiments.
Moreover, 5(6)-Carboxyfluorescein N-succinimidyl Ester also functions as a valuable component in assay development, acting as a fluorescence polarization immunoassay tracer. This capability allows for the quantification of various analytes, including the detection of specific drugs or antibodies, by measuring changes in fluorescence polarization upon molecular binding. The ability to buy this versatile compound for diverse applications highlights its broad impact on scientific research.
NINGBO INNO PHARMCHEM CO.,LTD. is dedicated to supporting the scientific community by supplying premium chemical products. Our commitment to quality ensures that researchers receive reliable fluorescent protein labeling agents and other essential reagents to advance their studies in protein function, cellular biology, and molecular diagnostics. The precision and sensitivity afforded by these tools are key to unlocking new biological discoveries.
The succinimidyl ester functionality of 5(6)-Carboxyfluorescein N-succinimidyl Ester makes it highly reactive towards primary amine groups, which are abundant in proteins, particularly on lysine residues and N-termini. This facile reaction allows for the efficient covalent attachment of the fluorescein moiety to the protein of interest. The resulting fluorescent protein conjugates can then be utilized in a variety of biochemical and cell biology applications. For example, researchers can prepare fluorescent derivatives of proteins like tubulin, enabling detailed studies of cytoskeletal dynamics using techniques such as fluorescence microscopy and flow cytometry.
The application of these biological fluorescent derivatives extends to investigating protein localization within different cellular compartments, tracking protein-protein interactions, and monitoring protein trafficking. The bright fluorescence of the fluorescein tag allows for sensitive detection, even at low protein concentrations. This is particularly important when studying rare proteins or transient cellular events. Researchers looking to buy fluorescent labeling agents can rely on the consistent quality offered by NINGBO INNO PHARMCHEM CO.,LTD. for these demanding experiments.
Moreover, 5(6)-Carboxyfluorescein N-succinimidyl Ester also functions as a valuable component in assay development, acting as a fluorescence polarization immunoassay tracer. This capability allows for the quantification of various analytes, including the detection of specific drugs or antibodies, by measuring changes in fluorescence polarization upon molecular binding. The ability to buy this versatile compound for diverse applications highlights its broad impact on scientific research.
NINGBO INNO PHARMCHEM CO.,LTD. is dedicated to supporting the scientific community by supplying premium chemical products. Our commitment to quality ensures that researchers receive reliable fluorescent protein labeling agents and other essential reagents to advance their studies in protein function, cellular biology, and molecular diagnostics. The precision and sensitivity afforded by these tools are key to unlocking new biological discoveries.
Perspectives & Insights
Core Pioneer 24
“Moreover, 5(6)-Carboxyfluorescein N-succinimidyl Ester also functions as a valuable component in assay development, acting as a fluorescence polarization immunoassay tracer.”
Silicon Explorer X
“This capability allows for the quantification of various analytes, including the detection of specific drugs or antibodies, by measuring changes in fluorescence polarization upon molecular binding.”
Quantum Catalyst AI
“The ability to buy this versatile compound for diverse applications highlights its broad impact on scientific research.”