Fast Blue B Salt (CAS 14263-94-6) is more than just a dye; it's a sophisticated chemical indicator crucial for many biochemical assays. Its utility stems from its molecular structure and its reactive properties, enabling it to serve as a reliable chromogenic substrate for detecting enzyme activity. Understanding its chemistry is key for researchers who need to buy this reagent with confidence.

Chemically, Fast Blue B Salt is a diazo dye. Its molecular formula, C14H12N4O2Cl2·ZnCl2, reveals it as a complex organic salt containing zinc and chloride ions. The diazo groups within its structure are particularly important for its function. In biochemical assays, these groups are often involved in coupling reactions. When an enzyme, such as alkaline phosphatase, acts upon a specific substrate, it releases a product that then reacts with the diazo groups of Fast Blue B Salt. This reaction forms a brightly colored, insoluble precipitate, typically a vibrant blue hue.

The intensity and localization of this colored precipitate directly correlate with the presence and activity of the target enzyme. This makes Fast Blue B Salt an excellent tool for quantitative and qualitative analysis of enzyme function. For example, in diagnostic kits or research protocols, the appearance of the blue color indicates a positive reaction, allowing scientists to confirm enzyme presence or measure enzyme kinetics.

The purity of Fast Blue B Salt is critical for its performance as an enzyme indicator. High purity ensures that the color reaction is specific to the enzymatic activity and not influenced by contaminants. Impure samples can lead to background staining or erroneous results, compromising experimental validity. Therefore, when purchasing Fast Blue B Salt, it is imperative to source it from reputable manufacturers who can guarantee high purity levels and provide detailed specifications.

As a manufacturer and supplier, we prioritize the quality and consistency of our Fast Blue B Salt. We understand that the accuracy of your biochemical assays depends on the reliability of your reagents. We invite researchers to purchase Fast Blue B Salt from us, knowing they are receiving a product manufactured under strict quality control. For inquiries about pricing, availability, and to place an order, please contact our sales team. We are committed to supporting your scientific research with high-quality chemical solutions.