In the realm of biochemical research, precise and sensitive detection methods are crucial for advancing scientific understanding. Chemiluminescence technology, particularly when employing advanced substrates, has become a cornerstone of many laboratory protocols. Among these, APS-5 (CAS 193884-53-6) stands out as a leading chemiluminescent substrate, widely adopted for its superior performance in assays involving alkaline phosphatase (AP).

At its core, APS-5 is a chemiluminescent substrate derived from 9,10-dihydroacridine. Its primary function is to serve as a reactant that, upon enzymatic activation by alkaline phosphatase, undergoes a chemical reaction producing light. This light emission is then detected and quantified, providing researchers with valuable data about the presence and concentration of specific molecules.

The utility of APS-5 in research laboratories is multifaceted, primarily stemming from its impressive performance characteristics. For instance, its high sensitivity allows researchers to detect very low concentrations of AP-labeled molecules. This is particularly important in applications such as ELISA and Western blotting, where the target analytes might be present in minute amounts. When you buy APS-5 from a reputable manufacturer like us, you are guaranteed a product that meets rigorous purity standards, ensuring reliable and reproducible results for your experiments.

Another significant advantage of APS-5 is its rapid signal generation. Research protocols often demand efficiency, and APS-5's ability to produce a strong luminescent signal quickly means that assay times can be considerably reduced. This allows for higher throughput in busy research environments and faster acquisition of critical experimental data. The sustained nature of the luminescence also means that experimental readings can be taken over an extended period, providing flexibility without compromising signal integrity.

Furthermore, the temperature insensitivity of APS-5 is a practical benefit for researchers. Many chemical reactions are highly dependent on precise temperature control. However, APS-5 exhibits stable luminescence across a practical temperature range (25-35°C), simplifying experimental setups and reducing the reliance on specialized, temperature-controlled equipment. This makes it an accessible and user-friendly reagent for a broad spectrum of laboratories.

The common applications of APS-5 in research include:

  • ELISA (Enzyme-Linked Immunosorbent Assay): For detecting antibodies, antigens, and proteins with high sensitivity and clear signal differentiation.
  • Western Blotting: For the sensitive detection of specific proteins after transfer to a membrane, allowing for clear visualization of protein bands.
  • Phosphatase Activity Assays: For directly measuring the activity of alkaline phosphatase in various biological samples.
  • Gene Chip Studies: As a luminescent probe to enhance detection capabilities in advanced genetic analysis.

For academic and industrial research labs, sourcing high-quality APS-5 is crucial for the success of their experiments. We, as a leading manufacturer and supplier, are committed to providing researchers with APS-5 that offers exceptional purity, competitive pricing, and a stable supply chain. Understanding how to leverage the unique properties of APS-5 can significantly enhance the outcomes of your research. If you are looking to purchase APS-5 or inquire about its price, our dedicated sales team is ready to assist you.