Electrophoresis Tracking: The Utility of Cresol Red (CAS 1733-12-6)
In molecular biology and biochemistry, electrophoresis is a cornerstone technique for separating and analyzing nucleic acids and proteins. Essential to the efficient execution of these methods is the use of tracking dyes, which provide visual confirmation of sample migration through the gel. Cresol Red (CAS 1733-12-6) is a well-established and highly useful tracking dye, and as a leading manufacturer and supplier in China, we are proud to offer high-quality Cresol Red to support your research needs.
Cresol Red, a synthetic triarylmethane dye, functions effectively as a tracking dye in various types of electrophoresis, including DNA, RNA, and protein separations. Unlike DNA ladders which are themselves the subject of analysis, tracking dyes are inert with respect to the separation process but are visible to the naked eye. Cresol Red provides a distinct color marker that moves through the gel matrix, allowing researchers to monitor the progress of the electrophoresis run in real-time. This visual feedback is crucial for determining the optimal time to terminate the experiment, preventing the over-separation or loss of analytes. Its migration characteristics are well-understood, making it a predictable component of electrophoresis protocols.
When used in agarose gels for DNA or RNA electrophoresis, Cresol Red typically migrates with an apparent molecular size comparable to that of a 125 bp DNA fragment. This makes it a convenient marker for estimating the migration front, especially when working with smaller DNA fragments. Importantly, Cresol Red has been shown not to inhibit common enzymatic reactions like Taq polymerase activity, meaning its presence in a sample will not interfere with downstream applications such as PCR. This compatibility is a significant advantage for researchers performing sequential experimental steps.
For laboratories and institutions requiring Cresol Red for their electrophoresis experiments, sourcing from a reliable manufacturer is key. Our company, based in China, specializes in producing high-purity Cresol Red (CAS 1733-12-6) with a minimum assay of 99%. This ensures that the dye performs consistently and predictably. We understand the demands of research and the importance of a stable supply chain. Procurement managers can benefit from our competitive pricing and our commitment to delivering quality reagents. We encourage you to contact us for a quote and to discuss your specific requirements.
In conclusion, Cresol Red (CAS 1733-12-6) is an essential reagent for electrophoresis, serving as a reliable tracking dye that aids in visualizing and controlling the separation process. Its compatibility with enzymatic reactions and predictable migration patterns make it a valuable tool for molecular biologists. We, as a dedicated China-based manufacturer and supplier, are focused on providing high-purity Cresol Red to meet the needs of the scientific community. Reach out to us today to secure a dependable source for this vital electrophoresis reagent.
Perspectives & Insights
Data Seeker X
“This visual feedback is crucial for determining the optimal time to terminate the experiment, preventing the over-separation or loss of analytes.”
Chem Reader AI
“Its migration characteristics are well-understood, making it a predictable component of electrophoresis protocols.”
Agile Vision 2025
“When used in agarose gels for DNA or RNA electrophoresis, Cresol Red typically migrates with an apparent molecular size comparable to that of a 125 bp DNA fragment.”