ABTS vs. TMB: Choosing the Right Peroxidase Substrate for Your Assay
When performing enzyme-linked assays, particularly those involving peroxidase enzymes like HRP, selecting the appropriate chromogenic substrate is crucial for optimal results. Two of the most commonly used substrates are ABTS (2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) and TMB (3,3',5,5'-Tetramethylbenzidine). Understanding their distinct characteristics can help researchers make an informed decision for their specific needs.
ABTS is known for its sensitivity and its ability to produce a soluble green end product that can be read spectrophotometrically at 405 nm. It is often favored in situations where high sensitivity is required or when dealing with samples that might produce high background noise. Its metastable cation formation contributes to its reliability in quantitative measurements.
TMB, on the other hand, typically produces a blue end product that turns yellow upon acidification. It is generally considered more sensitive than ABTS and is widely used in microwell ELISA formats. The reaction with TMB is usually stopped with an acid, resulting in a stable yellow product that is read at around 450 nm.
The choice between ABTS and TMB often depends on the specific requirements of the assay. For instance, if a soluble green product is preferred, or if dealing with high background, ABTS might be the better choice. If maximum sensitivity in microwell assays is the primary goal, TMB may be more suitable. Many researchers look to buy ABTS or TMB from reliable suppliers like NINGBO INNO PHARMCHEM CO.,LTD. to ensure product quality.
When considering the purchase of these substrates, it's important to note the availability from trusted manufacturers. NINGBO INNO PHARMCHEM CO.,LTD. provides both ABTS and other essential reagents for biochemical assays. Understanding the price points and availability of these compounds will assist in planning your experimental budget. Whether you choose to buy ABTS or TMB, ensuring the purity and consistency of the product is key to obtaining reproducible data. NINGBO INNO PHARMCHEM CO.,LTD. is a reliable source for these critical assay components.
ABTS is known for its sensitivity and its ability to produce a soluble green end product that can be read spectrophotometrically at 405 nm. It is often favored in situations where high sensitivity is required or when dealing with samples that might produce high background noise. Its metastable cation formation contributes to its reliability in quantitative measurements.
TMB, on the other hand, typically produces a blue end product that turns yellow upon acidification. It is generally considered more sensitive than ABTS and is widely used in microwell ELISA formats. The reaction with TMB is usually stopped with an acid, resulting in a stable yellow product that is read at around 450 nm.
The choice between ABTS and TMB often depends on the specific requirements of the assay. For instance, if a soluble green product is preferred, or if dealing with high background, ABTS might be the better choice. If maximum sensitivity in microwell assays is the primary goal, TMB may be more suitable. Many researchers look to buy ABTS or TMB from reliable suppliers like NINGBO INNO PHARMCHEM CO.,LTD. to ensure product quality.
When considering the purchase of these substrates, it's important to note the availability from trusted manufacturers. NINGBO INNO PHARMCHEM CO.,LTD. provides both ABTS and other essential reagents for biochemical assays. Understanding the price points and availability of these compounds will assist in planning your experimental budget. Whether you choose to buy ABTS or TMB, ensuring the purity and consistency of the product is key to obtaining reproducible data. NINGBO INNO PHARMCHEM CO.,LTD. is a reliable source for these critical assay components.
Perspectives & Insights
Nano Explorer 01
“Understanding their distinct characteristics can help researchers make an informed decision for their specific needs.”
Data Catalyst One
“ABTS is known for its sensitivity and its ability to produce a soluble green end product that can be read spectrophotometrically at 405 nm.”
Chem Thinker Labs
“It is often favored in situations where high sensitivity is required or when dealing with samples that might produce high background noise.”