For chemical intermediates like 2-Bromo-4-methoxyaniline (CAS: 32338-02-6), rigorous analytical characterization is fundamental to ensure its identity, purity, and suitability for downstream synthetic applications. NINGBO INNO PHARMCHEM CO.,LTD. employs a suite of advanced analytical techniques to guarantee the quality of its products.

Spectroscopic Techniques:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy: Both ¹H NMR and ¹³C NMR are critical for confirming the molecular structure. The characteristic signals for the aromatic protons, the methoxy group protons (~δ 3.8 ppm), and the amine protons provide a unique spectral fingerprint. Coupling patterns of aromatic protons and chemical shifts of carbon atoms help verify the substitution pattern and identify any isomeric impurities.
  • Mass Spectrometry (MS): High-Resolution Mass Spectrometry (HRMS) is employed to accurately determine the molecular weight of 2-Bromo-4-methoxyaniline (expected MW: 202.05 g/mol) and to confirm its elemental composition (C₇H₈BrNO). The presence of the bromine atom is readily identified by its characteristic isotopic pattern (¹⁷⁹Br and ¹⁸¹Br in an approximate 1:1 ratio), appearing as a pair of peaks separated by 2 mass units.
  • Fourier-Transform Infrared (FT-IR) Spectroscopy: FT-IR is used to identify key functional groups. Characteristic absorption bands for the primary amine (N-H stretches around 3400 cm⁻¹), the C-O stretch of the methoxy group (around 1230 cm⁻¹), and the C-Br stretch (around 600 cm⁻¹) are expected, confirming the presence of these functional moieties.

Chromatographic Techniques:

  • High-Performance Liquid Chromatography (HPLC): HPLC, often coupled with a Photodiode Array (PDA) detector, is essential for assessing the purity of 2-Bromo-4-methoxyaniline and quantifying impurities. Reversed-phase C18 columns with acetonitrile/water mobile phases are typically used to separate the target compound from related substances, with purity often exceeding 98.0%.
  • Gas Chromatography-Mass Spectrometry (GC-MS): GC-MS can be used for analyzing the purity of volatile derivatives or to identify volatile impurities present in the sample.

Other Characterization Methods:

  • Melting Point: While listed as a powder, derivatives or pure crystalline forms will have specific melting points that serve as an indicator of purity.
  • X-ray Crystallography: For solid-state structure determination, X-ray crystallography can provide definitive confirmation of molecular arrangement and purity, especially for crystalline derivatives.

NINGBO INNO PHARMCHEM CO.,LTD. utilizes these comprehensive analytical methods to ensure that every batch of 2-Bromo-4-methoxyaniline meets the highest standards, providing reliable and pure materials for critical synthetic applications in research and industry.