Understanding the complex mechanisms of enzymes and the intricate structures of cellular organelles is fundamental to advancing biological sciences. 2-Iminothiolane Hydrochloride (CAS 4781-83-3) has emerged as a key reagent in these fields, empowering researchers with its unique capabilities in protein modification, specifically through protein-protein cross-linking and the introduction of reactive sulfhydryl groups. For scientists looking to buy this product, its specific applications are worth detailed consideration.

In the study of enzymes, particularly oligomeric enzymes, 2-Iminothiolane Hydrochloride serves as an invaluable tool. By inducing cross-linking between subunits, researchers can stabilize enzyme complexes, investigate subunit interactions, and gain insights into the conformational changes that occur during enzymatic activity. This capability is crucial for elucidating enzyme mechanisms, understanding allosteric regulation, and determining the quaternary structure of enzyme systems. When you buy this chemical, you are investing in a means to deepen your understanding of enzyme function.

The investigation of cellular organelles also benefits significantly from the use of 2-Iminothiolane Hydrochloride. Within the complex cellular environment, identifying and analyzing protein interactions is vital for understanding organelle function and communication. The reagent's ability to cross-link proteins within specific cellular compartments or directly within isolated organelles allows for the capture and study of transient protein complexes. This aids in mapping protein-protein interaction networks within mitochondria, endoplasmic reticulum, or other critical cellular structures.

Furthermore, the introduction of sulfhydryl groups via 2-Iminothiolane Hydrochloride provides a reactive handle for further biochemical assays related to organelle or enzyme function. These sulfhydryl groups can be used for conjugation with specific labels or for immobilization strategies, facilitating targeted studies and the development of novel diagnostic or research tools. Ensuring a consistent supply of high-purity (e.g., 98%) 2-Iminothiolane Hydrochloride from a reliable manufacturer, such as those found in China, is essential for the success of these detailed studies.

In summary, the applications of 2-Iminothiolane Hydrochloride in enzyme and cellular organelle studies are profound. Its ability to facilitate protein cross-linking and introduce reactive sulfhydryl groups makes it an indispensable reagent for researchers seeking to unravel the complexities of biological systems. By strategically purchasing this chemical from trusted sources, scientists can equip themselves with the tools necessary for significant breakthroughs.