Biochemical assays are the bedrock of biological research, providing insights into enzymatic activity, protein interactions, and cellular processes. The success of these assays often hinges on maintaining precise environmental conditions, with pH control being paramount. MES Monohydrate (CAS 145224-94-8) is a frequently employed biological buffer that offers distinct advantages for a wide array of biochemical applications. As a leading manufacturer and supplier, we offer insights into how to effectively utilize MES Monohydrate and why sourcing from a reputable provider is crucial.

MES Monohydrate is classified as a Good's buffer, characterized by its zwitterionic structure and its effective buffering capacity in the pH range of 5.5 to 6.7. Its pKa value of approximately 6.1 at 20°C makes it exceptionally suitable for biological systems that operate optimally within this slightly acidic to neutral pH range. This makes it a preferred choice for enzyme kinetic studies, protein binding assays, and investigations involving pH-sensitive biological molecules.

Key properties that make MES Monohydrate invaluable in biochemical assays include its high water solubility, which allows for easy preparation of stock solutions, and its minimal UV absorbance, ensuring that it does not interfere with spectrophotometric measurements commonly used to track reaction progress. Furthermore, its low tendency to chelate metal ions is a significant advantage, preventing unintended interactions that could affect enzyme activity or experimental results. This inertness makes it ideal for experiments involving transition metals or sensitive protein structures.

To ensure the reliability and reproducibility of your biochemical assays, it is imperative to use high-quality MES Monohydrate. We supply MES Monohydrate with a guaranteed purity of ≥99.5%, produced under strict quality control measures. This high purity minimizes the presence of contaminants that could potentially skew assay results or inhibit biological processes.

For researchers seeking to optimize their biochemical assays, consider the following when working with MES Monohydrate:

  • Preparation: Prepare MES solutions using deionized or distilled water. Titrate with a strong base like NaOH to achieve the desired pH, if necessary, although it is often used in its pre-formulated acidic state for titration.
  • Storage: Store MES Monohydrate powder in a cool, dry place. Prepared solutions are generally stable at 2-8°C for extended periods, but it's always advisable to prepare fresh solutions or check for precipitates.
  • Compatibility: Be mindful of its buffering range and avoid using it in pH ranges where its buffering capacity is low.

If your research demands consistent and high-performance biological buffers, look no further. We are a premier MES Monohydrate supplier in China, offering competitive MES Monohydrate price for bulk and research quantities. When you need to buy MES Monohydrate for your critical biochemical assays, choose a partner committed to quality and scientific advancement.