In the competitive landscape of biochemical research and diagnostics, achieving high sensitivity in detection assays is crucial for accurate results and groundbreaking discoveries. Biotinyl Tyramide, a specialized chemical compound, has become a cornerstone reagent for enhancing signal amplification in a variety of critical laboratory techniques. Its primary application lies within Tyramide Signal Amplification (TSA), a method that dramatically increases the detectability of targets in biological samples. As a dedicated manufacturer and supplier, we are proud to offer high-purity Biotinyl Tyramide to researchers seeking to optimize their experimental outcomes. For those looking to buy Biotinyl Tyramide, our commitment to quality and competitive pricing makes us an excellent choice.

How Biotinyl Tyramide Powers Signal Amplification

Biotinyl Tyramide functions by working in concert with an enzyme, most commonly Horseradish Peroxidase (HRP). In a typical TSA protocol, the HRP enzyme, often conjugated to a specific antibody or probe, catalyzes the deposition of multiple biotin molecules onto nearby tyrosinated proteins in the sample. This enzymatic reaction generates a highly localized and concentrated 'cloud' of biotin around the initial binding site. This amplification effect means that even targets present in very low quantities can generate a strong, detectable signal. The amplified biotin signal is then visualized using a streptavidin conjugate, which can be labeled with a fluorescent molecule, an enzyme for chromogenic detection, or other detection modalities.

Key Applications and Benefits:

  • Immunohistochemistry (IHC): Biotinyl Tyramide is extensively used in IHC to enhance the detection of protein antigens in tissue sections, leading to clearer images and the ability to identify proteins expressed at low levels.
  • In Situ Hybridization (ISH): In ISH, it amplifies the signal from DNA or RNA probes, enabling more sensitive detection of specific genetic sequences within cells and tissues.
  • Fluorescence In Situ Hybridization (FISH): Similar to ISH, Biotinyl Tyramide boosts the fluorescent signal for enhanced visualization of chromosomal abnormalities or gene localization.
  • Other Immunoassays: The principle of signal amplification can be applied to various other immunoassay formats where increased sensitivity is beneficial.
  • Reduced Reagent Consumption: The amplified signal often allows researchers to use lower concentrations of primary antibodies or probes, potentially reducing costs and minimizing non-specific binding.

Your Trusted Supplier for Biotinyl Tyramide

The effectiveness of TSA is directly linked to the purity and quality of the Biotinyl Tyramide used. We ensure that our Biotinyl Tyramide (CAS 41994-02-9) meets rigorous purity standards, often exceeding 98%. This commitment to quality means you can rely on our product for consistent, reproducible results in your sensitive detection experiments. As a manufacturer and supplier based in China, we offer competitive pricing without compromising on the high performance expected by researchers worldwide. We are dedicated to supporting scientific advancement by providing reliable, high-quality reagents.

Partner with Us for Your Research Needs

Whether your research focuses on cancer biology, neuroscience, or molecular diagnostics, Biotinyl Tyramide can be a critical component in achieving your detection goals. We offer various packaging sizes to accommodate different laboratory needs. Our experienced technical support team is also available to provide guidance on optimal usage protocols and answer any questions you may have. By choosing us as your supplier, you gain access to a superior quality reagent and a partner committed to facilitating your research breakthroughs.