In the dynamic field of life science research, the ability to detect and visualize biomolecules with high sensitivity and specificity is critical for scientific discovery. Biotinyl Tyramide has become an indispensable reagent, primarily known for its role in Tyramide Signal Amplification (TSA). This technique significantly boosts the detection capabilities in essential research methods like immunohistochemistry (IHC) and in situ hybridization (ISH). As a leading supplier of specialized biochemicals, we offer premium Biotinyl Tyramide, empowering researchers worldwide to achieve clearer and more definitive results. If you're looking to buy Biotinyl Tyramide, our commitment to quality makes us your ideal partner.

The Mechanism of Signal Amplification with Biotinyl Tyramide

TSA works by utilizing an enzyme, typically horseradish peroxidase (HRP), to catalyze the deposition of multiple biotin molecules around a target molecule. When an HRP-conjugated antibody binds to its specific target, it initiates a reaction with Biotinyl Tyramide in the presence of hydrogen peroxide. This reaction generates a reactive biotinyl tyramide free radical that covalently attaches to nearby tyrosine residues in proteins. The result is a highly localized and significantly amplified biotin signal at the target site. This dense accumulation of biotin can then be readily detected using labeled streptavidin conjugates, such as those linked to fluorescent dyes or enzymes, providing a much stronger signal than would be achievable with a single antibody-enzyme conjugate alone.

Applications Across Life Sciences:

  • Protein Detection (IHC/ICC): In immunohistochemistry (IHC) and immunocytochemistry (ICC), Biotinyl Tyramide dramatically enhances the detection of proteins in tissue sections and cells, enabling the visualization of low-abundance proteins.
  • Nucleic Acid Detection (ISH/FISH): For in situ hybridization (ISH) and fluorescence in situ hybridization (FISH), Biotinyl Tyramide amplifies the signal from labeled probes, improving the detection sensitivity for specific DNA or RNA sequences.
  • Biotechnology Research: It serves as a crucial reagent in various biotechnology applications where amplified detection is needed, including certain types of immunoassays and cell-based assays.
  • Emerging Techniques: Biotinyl Tyramide is also being incorporated into newer techniques like APEX proximity labeling for mapping protein interactions and cellular localization.

Why Choose Our Biotinyl Tyramide?

The efficacy of TSA directly correlates with the purity of the Biotinyl Tyramide. We are a reputable manufacturer and supplier providing Biotinyl Tyramide (CAS 41994-02-9) with a guaranteed purity of over 98%. This ensures that your experiments yield reproducible and reliable results. By choosing us, you are opting for a high-quality reagent that minimizes background noise and maximizes signal intensity. Sourcing your research essentials from a trusted manufacturer in China offers a cost-effective solution without compromising on performance. We are committed to supporting your research endeavors with premium biochemicals.

Procuring Biotinyl Tyramide for Your Lab

Whether you are a seasoned researcher or just starting, integrating Biotinyl Tyramide into your protocols can significantly advance your detection capabilities. We offer Biotinyl Tyramide in various quantities to suit the needs of different projects, from small-scale experiments to large-scale studies. Our customer service and technical support teams are available to assist you with product selection, usage protocols, and any inquiries you may have. Partner with us for your Biotinyl Tyramide needs and experience the difference that quality and reliability can make in your life science research.