In the realm of molecular biology and biochemistry, gel electrophoresis is a cornerstone technique for separating and analyzing biomolecules like DNA and proteins. A critical component of this process is the use of tracking dyes, which provide visual cues for monitoring the progress of the electrophoresis run. Among the most widely used tracking dyes is Bromophenol Blue (CAS 115-39-9). As a key supplier of chemical reagents, understanding the application and sourcing of Bromophenol Blue is vital for researchers and procurement specialists.

The Function of Bromophenol Blue in Gel Electrophoresis

Bromophenol Blue, a synthetic dye with the chemical formula C19H10Br4O5S, serves multiple purposes in the laboratory. Its primary role in gel electrophoresis is to act as a visual marker. When mixed with DNA or protein samples loaded into the gel wells, it migrates through the gel matrix along with the sample molecules. This migration is influenced by factors such as the gel concentration, buffer composition, and the dye's molecular weight and charge. Typically, Bromophenol Blue migrates at a rate comparable to DNA fragments of around 300-500 base pairs in a standard 1% agarose gel. This allows researchers to visually track the progress of the electrophoresis run and determine when to stop the process, thereby preventing the molecules of interest from migrating off the end of the gel.

Key Characteristics for Electrophoresis Use

  • Visual Tracking: Its distinct blue color at neutral to alkaline pH provides a clear visual marker.
  • Migration Rate: It migrates at a predictable rate, allowing for estimation of separation progress.
  • Compatibility: It is compatible with common electrophoresis buffers and gel types (agarose and polyacrylamide).
  • pH Indication: While primarily a tracking dye, its pH indicator properties (transition from yellow to blue between pH 3.0 and 4.6) can also be relevant in certain buffer systems.

Where to Source High-Quality Bromophenol Blue

For researchers and institutions, securing a reliable source of high-purity Bromophenol Blue is crucial for consistent and accurate experimental results. We are a reputable bromophenol blue manufacturer and supplier, offering a product that meets stringent quality standards essential for molecular biology applications. When you buy bromophenol blue from us, you are assured of a product that performs reliably as a tracking dye. We provide competitive bromophenol blue price options, especially for bulk purchases, making it an accessible reagent for academic and research laboratories. As a leading bromophenol blue supplier in China, we understand the importance of timely delivery and consistent quality. Explore our offerings and find the best bromophenol blue for electrophoresis to enhance your molecular separation experiments.

In summary, Bromophenol Blue is an indispensable tool in gel electrophoresis, providing crucial visual guidance for researchers. By understanding its function and sourcing it from a trusted supplier, scientists can ensure the success of their experiments and gain valuable insights into molecular separations.