CAPSO Sodium Salt: Optimizing Protein Transfer in Electrophoresis
Electrophoresis is a cornerstone technique in molecular biology and biochemistry, enabling the separation and analysis of proteins. A critical step in many electrophoretic protocols, particularly Western blotting, is the efficient transfer of proteins from a gel matrix to a membrane. This process is highly dependent on the transfer buffer's composition and pH. CAPSO Sodium Salt (CAS 102601-34-3) has emerged as a preferred buffer for achieving optimal protein transfer, especially for proteins that are difficult to transfer at standard pH levels.
Traditional transfer buffers, often based on Tris-Glycine, operate at a neutral to slightly alkaline pH. While effective for many proteins, they can exhibit limited efficiency in transferring strongly basic proteins. CAPSO Sodium Salt, a zwitterionic buffer, allows for the formulation of transfer buffers at significantly higher pH ranges, typically between 8.9 and 10.3. At these alkaline conditions, proteins with higher isoelectric points become more negatively charged, facilitating a more effective transfer onto the membrane. This makes CAPSO Sodium Salt an indispensable component for researchers focusing on the analysis of such proteins.
The selection of a high-quality buffer is crucial for reproducible results. As a dedicated manufacturer and supplier, we ensure that our CAPSO Sodium Salt meets stringent purity standards (≥99.0%). This high purity, coupled with its excellent water solubility and low metal ion binding, guarantees consistent performance in your electrophoresis setups. When you choose to buy CAPSO Sodium Salt from us, you are ensuring the integrity of your experimental workflow.
For laboratories involved in protein identification, sequencing, and various blotting techniques, incorporating CAPSO Sodium Salt into their buffer systems can significantly improve the efficiency and completeness of protein transfer. Its unique chemical properties make it a superior choice for challenging protein transfer scenarios. We are a trusted supplier in China, providing this essential reagent for research and industrial applications. If you are looking to purchase CAPSO Sodium Salt, we offer competitive pricing and reliable supply. Reach out to us for a quotation and learn how our commitment to quality can benefit your research.
Perspectives & Insights
Nano Explorer 01
“If you are looking to purchase CAPSO Sodium Salt, we offer competitive pricing and reliable supply.”
Data Catalyst One
“Reach out to us for a quotation and learn how our commitment to quality can benefit your research.”
Chem Thinker Labs
“Electrophoresis is a cornerstone technique in molecular biology and biochemistry, enabling the separation and analysis of proteins.”