Diethyl Pyrocarbonate (DEPC), scientifically known as diethyl dicarbonate and identified by CAS number 1609-47-8, is a compound of significant interest in both biological research and chemical synthesis. While its fame largely stems from its ability to inactivate RNases, understanding its underlying chemical properties reveals its broader utility as a fine chemical. NINGBO INNO PHARMCHEM CO.,LTD. is a key supplier of DEPC, and we aim to provide a deeper understanding of this multifaceted compound.

Chemically, DEPC is an ester of pyrocarbonic acid. Its molecular formula is C6H10O5, and it presents as a clear, colorless liquid. A defining characteristic of DEPC is its reactivity towards nucleophiles. This reactivity is exploited in its primary laboratory application: reacting with nucleophilic groups in enzymes like histidine, lysine, and cysteine residues within RNases. This reaction effectively neutralizes the enzyme, preventing it from degrading RNA. The chemical properties of diethyl pyrocarbonate dictate its efficacy and also its limitations, such as its instability in aqueous solutions over time, where it undergoes hydrolysis to ethanol and carbon dioxide.

In the realm of chemical synthesis, DEPC serves as a valuable reagent and intermediate. Its electrophilic nature allows it to participate in acylation reactions, specifically ethoxycarbonylation. This means it can be used to introduce the ethoxycarbonyl group (-COOEt) onto alcohols, amines, and thiols, forming carbonates, carbamates, and thiocarbonates, respectively. These functional groups are important building blocks in the synthesis of pharmaceuticals, agrochemicals, and specialty polymers. As a fine chemical, its controlled reactivity makes it a sought-after component in precise organic syntheses.

The compound's physical properties also contribute to its handling and application. With a relatively low boiling point and solubility in organic solvents, it can be easily incorporated into reaction mixtures. However, its susceptibility to hydrolysis necessitates careful storage and handling, typically in tightly sealed containers away from moisture. Understanding these chemical properties of diethyl pyrocarbonate is essential for successful outcomes, whether preparing nuclease-free water or utilizing it in complex synthetic pathways.

NINGBO INNO PHARMCHEM CO.,LTD. is committed to supplying high-purity DEPC, ensuring that researchers and chemists have access to a reliable product for their diverse needs. Whether for the critical DEPC RNase inactivation protocol or for advanced chemical synthesis, our DEPC meets the necessary quality standards. We invite you to explore the chemical versatility of DEPC and how our supply can support your scientific and industrial objectives.