The Chemistry Behind HDAOS Sodium Salt: A Key Chromogenic Substrate
Understanding the chemical properties of key reagents is fundamental for anyone involved in biochemical research or diagnostic assay development. HDAOS Sodium Salt (CAS 82692-88-4), a sophisticated molecule often referred to as a Trinder's reagent, serves as a critical chromogenic substrate. As a leading supplier of these specialized chemicals, we aim to provide insight into why HDAOS is so valuable and what qualities researchers should look for when they need to buy HDAOS Sodium Salt.
Chemically, HDAOS is N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt. Its structure features an aniline backbone substituted with methoxy groups, a hydroxyl group, and a sulfopropyl moiety, all contributing to its desirable properties. The sulfopropyl group, in particular, imparts excellent water solubility, which is a significant advantage in aqueous assay systems. This high solubility ensures that the reagent dissolves readily and disperses evenly, facilitating efficient reactions and minimizing aggregation issues.
The chromogenic aspect of HDAOS comes into play during oxidation reactions. In assays involving enzymes that produce hydrogen peroxide (H₂O₂), HDAOS can act as a coupler. When catalyzed by peroxidase, H₂O₂ oxidizes HDAOS, leading to the formation of a colored product. This colorimetric detection is highly sensitive and can be quantified using spectrophotometry. This principle is widely applied in clinical chemistry for determining the levels of various analytes, such as glucose and cholesterol. The reaction is typically facilitated by the presence of another reagent, like 4-aminoantipyrine (4-AAP), to produce a stable, intense color.
For laboratory professionals and manufacturers, sourcing HDAOS Sodium Salt from a reputable manufacturer ensures access to a product with high purity, often exceeding 99%. This level of purity is crucial because impurities can interfere with the sensitive colorimetric reactions, leading to inaccurate results or assay failure. Furthermore, the stability of the synthesized compound is vital for both storage and reaction performance. Our commitment as a China-based supplier is to deliver a product that consistently meets these demanding chemical specifications.
The selection of a reliable chromogenic substrate supplier is key to successful research and diagnostics. When you plan to purchase HDAOS Sodium Salt, consider its specific role in your assay. Its sensitivity, coupled with good stability and water solubility, makes it an excellent choice for quantitative analysis. We are dedicated to providing the scientific community with the high-quality reagents needed to drive innovation and improve diagnostic accuracy. For inquiries regarding HDAOS reagent price or bulk orders, please contact us.
Perspectives & Insights
Quantum Pioneer 24
“When catalyzed by peroxidase, H₂O₂ oxidizes HDAOS, leading to the formation of a colored product.”
Bio Explorer X
“This colorimetric detection is highly sensitive and can be quantified using spectrophotometry.”
Nano Catalyst AI
“This principle is widely applied in clinical chemistry for determining the levels of various analytes, such as glucose and cholesterol.”