In the realm of biochemical and biophysical research, maintaining precise pH control is paramount. Biological buffers play a critical role in ensuring the stability and functionality of enzymes, proteins, and other biomolecules. Among the array of available buffers, CAPSO Buffer (CAS 73463-39-5) stands out for its unique properties and broad applicability. This article delves into why CAPSO Buffer is an excellent choice for researchers and where to buy high-quality CAPSO Buffer from a reliable manufacturer.

Understanding CAPSO Buffer: Properties and Advantages

CAPSO, chemically known as 3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid, is a zwitterionic buffer that operates effectively within the alkaline pH range of 8.9 to 10.3. This specific pH range is crucial for numerous enzymatic reactions and cellular processes that function optimally in slightly alkaline conditions. What sets CAPSO apart is its negligible metal ion binding capacity, making it an invaluable tool when studying metal-dependent enzymes or reactions where metal ions are crucial catalysts or inhibitors. This characteristic minimizes interference, leading to more accurate experimental results. Furthermore, CAPSO exhibits good water solubility, simplifying the preparation of buffer solutions. Researchers often seek this specific buffer for applications such as protein electrotransfer, where it aids in the efficient transfer of proteins to membranes like nitrocellulose or PVDF during Western blotting and related techniques.

Applications Where CAPSO Buffer Excels

The versatility of CAPSO Buffer makes it a staple in various laboratory settings. Its primary applications include:

  • Biochemical Assays: For enzymatic studies that require a stable pH above 8.5, CAPSO provides a reliable buffering system.
  • Electrophoresis and Protein Transfer: CAPSO is frequently used as a buffer component in electrophoresis and, notably, in the electrotransfer of proteins to membranes. This application is critical for techniques like Western blotting, facilitating downstream detection and analysis. If you are looking to buy CAPSO Buffer for your protein analysis workflows, ensure you source from a reputable supplier.
  • Cell Culture Media: While HEPES and Tris are more common, CAPSO can be employed in specific cell culture media formulations where its higher pH buffering capacity is required.
  • Diagnostic Reagent Production: The consistent purity and performance of CAPSO make it a valuable component in the formulation of diagnostic reagents, where precision and reliability are non-negotiable. Manufacturers seeking stable buffer components will find CAPSO an excellent option.

Choosing a CAPSO Buffer Supplier

When selecting a supplier for CAPSO Buffer (CAS 73463-39-5), several factors are critical: product purity, consistency, availability, and pricing. As a leading manufacturer and supplier of fine chemicals, we are dedicated to providing high-quality CAPSO Buffer that meets stringent industry standards. Our commitment to research and development, coupled with robust manufacturing processes, ensures that you receive a product that performs exceptionally. We understand the importance of reliable supply chains for research institutions and diagnostic companies. Therefore, we focus on delivering consistent quality and competitive pricing. If you are in need of CAPSO Buffer for your laboratory or production needs, we encourage you to inquire about our bulk purchase options and competitive price quotes. As a trusted supplier, we aim to support your scientific endeavors with our premium biochemical reagents.

In conclusion, CAPSO Buffer (CAS 73463-39-5) is an indispensable tool for researchers working in alkaline pH ranges. Its unique properties, including minimal metal binding and excellent solubility, make it superior for various critical applications. By choosing to buy CAPSO Buffer from a reputable manufacturer and supplier, you ensure the accuracy and reproducibility of your scientific experiments and the quality of your diagnostic products. Contact us today to learn more or to request a sample and quote for your CAPSO Buffer requirements.