In the dynamic field of life sciences, precise and sensitive measurement tools are paramount. Coelenterazine, a vital luciferin substrate, stands at the forefront of bioluminescence technologies, enabling groundbreaking research across various disciplines. For scientists and procurement managers seeking to buy Coelenterazine, understanding its nuances and sourcing it from a reliable manufacturer is key to achieving accurate and reproducible results.

Coelenterazine (CAS 55779-48-1) is a naturally occurring molecule renowned for its role as a light-emitting substrate in bioluminescent reactions. Its primary function is to provide the light energy when oxidized by luciferases, enzymes found in organisms ranging from marine invertebrates to bacteria. This biochemical property makes it an indispensable component in numerous research applications.

The utility of Coelenterazine spans across several critical areas in biological research:

  • Reporter Gene Assays: Coelenterazine is extensively used with luciferase enzymes, such as Renilla luciferase and Gaussia luciferase, to monitor gene expression. By attaching a luciferase gene to a promoter of interest, researchers can quantify transcriptional activity through light emission, providing insights into gene regulation and cellular responses.
  • Bioluminescence Imaging (BLI): In preclinical research and cellular imaging, Coelenterazine allows for non-invasive visualization of biological processes in living organisms or cells. This capability is crucial for tracking disease progression, drug efficacy, and cellular localization.
  • Calcium Sensing: When complexed with photoproteins like aequorin, Coelenterazine emits light in response to calcium ions. This allows for sensitive real-time monitoring of intracellular calcium fluctuations, which are fundamental to cell signaling, muscle contraction, and neurological activity.
  • Chemiluminescence Detection: Coelenterazine can also be used in chemiluminescent assays to detect reactive oxygen species (ROS) and reactive nitrogen species (RNS), such as superoxide anion and peroxynitrite, offering a sensitive method for assessing oxidative stress.

When selecting Coelenterazine, purity is a non-negotiable factor. Impurities can lead to background luminescence, reduced signal-to-noise ratios, and inaccurate quantification. Leading suppliers, particularly those operating as manufacturers in China, offer Coelenterazine with high purity levels (often >98%) to meet the rigorous demands of scientific research. These manufacturers are adept at synthesizing and purifying Coelenterazine, ensuring lot-to-lot consistency.

For procurement managers, identifying a dependable supplier involves looking for companies that provide comprehensive technical support, timely delivery, and competitive pricing. Understanding the chemical structure and properties, such as its CAS number (55779-48-1), is also important for regulatory compliance and accurate inventory management.

In conclusion, whether you are performing advanced reporter gene assays, in vivo imaging, or cellular signaling studies, choosing high-quality Coelenterazine is vital. By partnering with a reputable manufacturer and supplier, you can ensure the success of your experiments and contribute to cutting-edge scientific discoveries. We invite you to request a quote for our premium Coelenterazine and experience the difference that quality and expertise make.