Understanding Diethyl Maleate's Role as a Glutathione Depleting Agent
Diethyl Maleate (CAS 141-05-9) plays a significant role in biological and biochemical research, primarily due to its function as a glutathione-depleting agent. Glutathione (GSH) is a tripeptide that acts as a critical antioxidant in cells, protecting them from damage caused by reactive oxygen species and other electrophilic compounds. By understanding how Diethyl Maleate affects glutathione levels, researchers can delve deeper into cellular defense mechanisms and the pathways involved in oxidative stress.
The mechanism by which Diethyl Maleate depletes glutathione involves its reaction with cellular thiols. As an alpha,beta-unsaturated compound, it readily undergoes a Michael addition with the sulfhydryl group of cysteine residues in glutathione. This conjugation effectively removes free glutathione from the cellular pool, impacting the cell's antioxidant capacity and its ability to detoxify harmful substances.
Researchers utilize Diethyl Maleate to experimentally induce oxidative stress in cellular or animal models. By reducing glutathione levels, they can observe the downstream effects on various cellular processes, including signal transduction pathways, protein function, and cell viability. This approach helps in understanding the physiological and pathological consequences of GSH depletion and identifying potential therapeutic targets.
For instance, studies using Diethyl Maleate have investigated its effects on NF-kappa B activation, cellular apoptosis, and the modulation of protein synthesis. These investigations provide valuable insights into the complex interplay between redox status and cellular function, contributing to our understanding of diseases associated with oxidative stress, such as neurodegenerative disorders and cancer.
NINGBO INNO PHARMCHEM CO.,LTD., as a dedicated supplier, provides high-purity Diethyl Maleate for these critical research applications. Researchers worldwide can rely on our products to accurately study the intricate mechanisms of cellular defense and oxidative stress, furthering scientific discovery in the biological sciences.
The mechanism by which Diethyl Maleate depletes glutathione involves its reaction with cellular thiols. As an alpha,beta-unsaturated compound, it readily undergoes a Michael addition with the sulfhydryl group of cysteine residues in glutathione. This conjugation effectively removes free glutathione from the cellular pool, impacting the cell's antioxidant capacity and its ability to detoxify harmful substances.
Researchers utilize Diethyl Maleate to experimentally induce oxidative stress in cellular or animal models. By reducing glutathione levels, they can observe the downstream effects on various cellular processes, including signal transduction pathways, protein function, and cell viability. This approach helps in understanding the physiological and pathological consequences of GSH depletion and identifying potential therapeutic targets.
For instance, studies using Diethyl Maleate have investigated its effects on NF-kappa B activation, cellular apoptosis, and the modulation of protein synthesis. These investigations provide valuable insights into the complex interplay between redox status and cellular function, contributing to our understanding of diseases associated with oxidative stress, such as neurodegenerative disorders and cancer.
NINGBO INNO PHARMCHEM CO.,LTD., as a dedicated supplier, provides high-purity Diethyl Maleate for these critical research applications. Researchers worldwide can rely on our products to accurately study the intricate mechanisms of cellular defense and oxidative stress, furthering scientific discovery in the biological sciences.
Perspectives & Insights
Bio Analyst 88
“Diethyl Maleate (CAS 141-05-9) plays a significant role in biological and biochemical research, primarily due to its function as a glutathione-depleting agent.”
Nano Seeker Pro
“Glutathione (GSH) is a tripeptide that acts as a critical antioxidant in cells, protecting them from damage caused by reactive oxygen species and other electrophilic compounds.”
Data Reader 7
“By understanding how Diethyl Maleate affects glutathione levels, researchers can delve deeper into cellular defense mechanisms and the pathways involved in oxidative stress.”