Disodium 4-Nitrophenyl Phosphate: A Key Reagent for Enzyme Assays
Enzyme assays are fundamental to understanding biological processes, developing diagnostics, and advancing pharmaceutical research. Among the most crucial reagents in this field is Disodium 4-Nitrophenyl Phosphate (PNPP), a substrate widely employed to detect the activity of alkaline phosphatase (AP). PNPP (CAS 4264-83-9) is prized for its ability to undergo enzymatic hydrolysis, producing a quantifiable chromogenic signal. As a premier manufacturer and supplier of high-quality chemical intermediates, NINGBO INNO PHARMCHEM CO.,LTD. is dedicated to providing PNPP that meets the rigorous demands of modern scientific applications.
The mechanism is elegantly simple: alkaline phosphatase cleaves the phosphate group from PNPP, releasing p-nitrophenol. This molecule, under alkaline conditions, turns yellow and can be measured spectrophotometrically, typically at wavelengths around 405 nm. This colorimetric conversion allows researchers to accurately determine the presence and activity level of alkaline phosphatase in various biological samples. Whether in diagnostic kits, like ELISA, or in fundamental research for enzyme kinetics, the reliability of PNPP is paramount.
For procurement managers and researchers looking to source this vital reagent, NINGBO INNO PHARMCHEM CO.,LTD. offers a compelling solution. Based in China, we operate as a reliable manufacturer and supplier, ensuring consistent quality and availability of Disodium 4-Nitrophenyl Phosphate. Our commitment to excellence means that we provide a product that is pure, stable, and performs optimally in diverse assay conditions. We understand the importance of cost-effectiveness, especially for bulk purchases, and strive to offer competitive pricing to support your ongoing research and development efforts.
We encourage you to consider NINGBO INNO PHARMCHEM CO.,LTD. as your trusted source for Disodium 4-Nitrophenyl Phosphate. Our extensive experience in chemical manufacturing and supply chain management ensures that you receive a product that meets your exacting standards. Please feel free to contact us for a quotation or to request a sample of our PNPP. We are confident that our high-quality product and dedicated service will meet your needs for critical enzyme assays.
Perspectives & Insights
Silicon Analyst 88
“This colorimetric conversion allows researchers to accurately determine the presence and activity level of alkaline phosphatase in various biological samples.”
Quantum Seeker Pro
“Whether in diagnostic kits, like ELISA, or in fundamental research for enzyme kinetics, the reliability of PNPP is paramount.”
Bio Reader 7
“For procurement managers and researchers looking to source this vital reagent, NINGBO INNO PHARMCHEM CO.”