For scientists and researchers, the correct application of specialized reagents is often the difference between groundbreaking discovery and inconclusive results. 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside (CAS 7240-90-6) is a prime example of such a crucial compound, widely recognized for its efficacy as a chromogenic substrate for detecting beta-galactosidase activity. Understanding its properties and optimal usage is key for any researcher looking to buy and utilize this powerful tool.

As a leading chemical manufacturer in China, NINGBO INNO PHARMCHEM CO.,LTD. is dedicated to providing not only high-purity products but also valuable insights to our clients. 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside is a white powder characterized by its molecular weight of 408.63. Its typical use involves its solubility in 100 mM DMSO, forming a stable solution ready for enzymatic reaction. When beta-galactosidase acts upon it, the resulting blue precipitate is a clear indicator, essential for applications in molecular biology, microbiology, and diagnostics.

When you choose to buy this enzyme substrate, consider the experimental context. For instance, in molecular cloning, the blue-white screening method relies on this compound to identify bacterial colonies that have successfully incorporated a recombinant plasmid. The presence of functional lacZ gene leads to beta-galactosidase production, resulting in blue colonies, while colonies lacking the functional gene remain white. Precise execution and reagent quality are paramount for accurate screening.

Furthermore, maintaining the integrity of the reagent is crucial. Proper storage, typically in a cool, dry place, away from direct sunlight, will ensure its stability. When preparing solutions, dissolving it in a suitable solvent like DMSO is recommended. Researchers often seek to buy from suppliers who can guarantee consistency between batches, ensuring reproducibility in their long-term studies. As your trusted chemical supplier, we ensure that every batch of CAS 7240-90-6 meets rigorous quality control standards.

In conclusion, 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside is more than just a chemical; it's a vital component for unlocking scientific understanding. By leveraging expert knowledge on its application and purchasing from reliable manufacturers like us, researchers can maximize its potential and achieve dependable results in their critical work.