Biochemical assays demand precision and sensitivity, especially when quantifying biomolecules present in minute quantities. O-Phthalaldehyde (OPA) has emerged as a cornerstone reagent in many such assays, primarily due to its ability to react with primary amines to produce highly fluorescent products. This property makes it invaluable for detecting and quantifying amino acids, peptides, and proteins in various biological samples.

As a premier O-Phthalaldehyde supplier, we provide high-purity OPA specifically formulated for biochemical applications. Our commitment is to equip researchers with the tools they need for accurate analysis. When you buy OPA from us, you can be confident in its performance for sensitive assays, whether you are working in a research laboratory or a clinical diagnostic setting.

The fundamental reaction involves OPA with a thiol (like 2-mercaptoethanol) and a primary amine. This reaction, typically carried out in aqueous buffers, yields a fluorescent derivative with strong emission in the visible spectrum. This fluorescence can then be measured using spectrofluorometers or integrated into analytical platforms like HPLC or flow cytometry. The sensitivity achieved with OPA is remarkable, often allowing for the detection of picomole quantities of target analytes, significantly outperforming many older derivatization methods.

OPA's applications in biochemical assays are diverse. It is widely used for:

  • Quantifying amino acids in protein hydrolysates.
  • Measuring peptide and protein concentrations in biological fluids.
  • Detecting histamine and histidine, crucial for food safety and clinical diagnostics.
  • Assaying cholesterol in the picomole range.

For professionals seeking to optimize their assays, sourcing high-quality OPA is paramount. We offer competitive O-Phthalaldehyde price in China, making this advanced analytical reagent accessible. As a dedicated O-Phthalaldehyde manufacturer, we understand the critical nature of your work. Whether you need OPA for pre-column derivatization in HPLC, flow cytometric measurements, or other specialized assays, we are your reliable source.

We invite researchers and laboratory managers to explore our range of OPA products. To discuss your specific assay requirements or to obtain a quotation for our OPA reagents, please contact our customer service team. Partner with us to enhance the sensitivity and accuracy of your biochemical analyses.