In the realm of biochemistry and food science, accurate amino acid profiling is fundamental. High-Performance Liquid Chromatography (HPLC) is the workhorse for this analysis, and Phenyl Isothiocyanate (PITC) plays a critical, often unsung, role. As a leading supplier of fine chemicals, we understand the importance of high-quality reagents like PITC for achieving precise analytical results.

Phenyl Isothiocyanate (CAS 103-72-0) is widely recognized for its utility as a derivatizing agent. Its primary function in HPLC amino acid analysis is to react with the free amino groups of amino acids, forming stable phenylthiocarbamyl (PTC) derivatives. These derivatives possess distinct chromatographic properties and UV absorption characteristics that make them ideal for sensitive detection by HPLC. Without this derivatization step, many amino acids would not be adequately separated or detected by standard HPLC methods.

The process involves reacting a sample containing amino acids with PITC, typically under alkaline conditions. This reaction yields PTC-amino acids, which are then injected into the HPLC system. The separation and subsequent detection provide a quantitative breakdown of the amino acid composition of the original sample. This is invaluable for researchers in nutritional science, pharmaceutical development, and quality control for food products. The ability to accurately buy PITC from a reliable manufacturer ensures the consistency and reproducibility of these critical analyses.

For procurement managers and lab scientists, selecting the right supplier for PITC is a strategic decision. A supplier based in China, with expertise in fine chemical manufacturing, can offer high-purity PITC (≥97%) essential for sensitive chromatographic applications. Ensuring the reagent meets the specifications required for derivatization, such as low impurity levels that could interfere with detection, is crucial. Engaging with suppliers who provide comprehensive technical documentation and responsive customer support facilitates seamless integration of PITC into your laboratory workflows.

The demand for accurate amino acid analysis continues to grow across various industries. By leveraging the capabilities of Phenyl Isothiocyanate and partnering with a dependable chemical manufacturer, laboratories can achieve greater accuracy and efficiency in their analytical endeavors, driving scientific discovery and product innovation.