Mastering Amino Acid Enantiomeric Purity with HPLC Derivatization
In the realm of pharmaceuticals, biotechnology, and nutritional science, the precise determination of enantiomeric purity for amino acids is paramount. Chirality, the property of molecules that makes them non-superimposable on their mirror images, profoundly impacts biological activity. For L-amino acids and their D-counterparts, differences in efficacy and safety profiles necessitate rigorous analytical control. High-Performance Liquid Chromatography (HPLC) coupled with chiral derivatization stands as a cornerstone technique for achieving this critical separation and quantification. This is where specialized reagents become indispensable.
One such vital reagent is Nα-(2,4-Dinitro-5-fluorophenyl)-L-valinamide, identified by its CAS number 132679-61-9. This compound acts as a chiral derivatizing agent, reacting with amino acids to form diastereomeric derivatives. These derivatives possess distinct physical and chemical properties, allowing for their separation on standard reversed-phase HPLC columns. As a leading manufacturer and supplier in China, we are committed to providing researchers and quality control laboratories with a consistently high-purity grade of this essential compound. When you buy Nα-(2,4-Dinitro-5-fluorophenyl)-L-valinamide from us, you invest in accuracy and reliability.
The process typically involves reacting the amino acid mixture with Nα-(2,4-Dinitro-5-fluorophenyl)-L-valinamide under controlled conditions. The resulting derivatives are then analyzed by HPLC, often with UV detection due to the chromophoric nature of the dinitrophenyl moiety. The differing retention times of the diastereomers on the column allow for their quantification, thereby revealing the enantiomeric ratio of the original amino acid sample. This technique is invaluable for ensuring the quality of active pharmaceutical ingredients (APIs), monitoring fermentation processes, and verifying the composition of dietary supplements.
For procurement professionals and scientists seeking to purchase chiral derivatizing agents, understanding the importance of reagent quality cannot be overstated. Impurities in the derivatizing agent can lead to spurious peaks, inaccurate quantifications, and compromised analytical results. Our commitment as a dedicated manufacturer ensures that our Nα-(2,4-Dinitro-5-fluorophenyl)-L-valinamide meets stringent purity standards, typically exceeding 98.0% as determined by HPLC. This high level of purity translates directly to more robust and reproducible analytical outcomes.
We understand that supply chain stability and cost-effectiveness are crucial considerations. As a well-established supplier, we offer competitive pricing for Nα-(2,4-Dinitro-5-fluorophenyl)-L-valinamide, making it accessible for both research laboratories and large-scale industrial applications. Whether you require small quantities for method development or bulk orders for routine analysis, our efficient production and logistics ensure timely delivery. If you are in the market to buy CAS 132679-61-9, consider partnering with us for a dependable and economical solution. Feel free to reach out to our sales team for a personalized quote and to discuss your specific requirements.
In conclusion, the accurate assessment of amino acid enantiomeric purity is fundamental to many scientific and industrial disciplines. Nα-(2,4-Dinitro-5-fluorophenyl)-L-valinamide, when sourced from a reliable manufacturer like us, is a powerful tool for achieving this. Our dedication to quality, competitive pricing, and robust supply chain makes us the preferred supplier for this critical analytical reagent. Contact us today to explore how our Nα-(2,4-Dinitro-5-fluorophenyl)-L-valinamide can enhance your analytical capabilities.
Perspectives & Insights
Data Seeker X
“Feel free to reach out to our sales team for a personalized quote and to discuss your specific requirements.”
Chem Reader AI
“In conclusion, the accurate assessment of amino acid enantiomeric purity is fundamental to many scientific and industrial disciplines.”
Agile Vision 2025
“Nα-(2,4-Dinitro-5-fluorophenyl)-L-valinamide, when sourced from a reliable manufacturer like us, is a powerful tool for achieving this.”