For researchers in the life sciences, understanding the intricacies of bioluminescent assays is paramount. At the heart of many such experiments lies D-Luciferin Potassium Salt (CAS 115144-35-9), a critical substrate that fuels the light-producing reaction catalyzed by firefly luciferase. As a discerning buyer, sourcing high-purity D-Luciferin Potassium Salt is key to achieving reliable and sensitive results in applications ranging from reporter gene expression studies to ATP detection. This guide aims to equip you with the knowledge to make informed purchasing decisions.

Why Choose D-Luciferin Potassium Salt?

D-Luciferin Potassium Salt is the water-soluble form of D-luciferin, the natural substrate found in fireflies. When combined with luciferase, ATP, and oxygen, it undergoes an enzymatic reaction that emits light at approximately 560 nm. This chemiluminescent reaction is highly efficient and can be precisely quantified, making it an invaluable tool in numerous scientific disciplines. Its ability to generate strong light signals with low background noise makes it particularly suitable for detecting low levels of gene expression or trace amounts of ATP.

Key Applications for Buyers:

  • Reporter Gene Assays: D-Luciferin Potassium Salt is indispensable for monitoring gene expression. Researchers can link the luciferase gene to a promoter of interest. By adding D-Luciferin Potassium Salt, the resulting light output directly correlates with the promoter's activity, providing a quantitative measure of gene transcription. This is crucial in areas like drug discovery and understanding cellular pathways. If you are looking to buy D-Luciferin Potassium Salt for reporter gene assays, focus on suppliers offering high purity grades.
  • ATP Measurement: The luciferase system's dependence on ATP makes D-Luciferin Potassium Salt an excellent tool for quantifying intracellular or extracellular ATP levels. This is vital for assessing cellular energy status, viability, and metabolic activity. When considering D-Luciferin Potassium Salt for ATP detection, consistency in product performance is paramount.
  • Bioluminescence Imaging (BLI): In vivo and in vitro BLI techniques utilize the light generated by luciferase-luciferin reactions to track biological processes, such as tumor growth, infection spread, or gene delivery, within living organisms or cells. For such demanding applications, sourcing from a reputable D-Luciferin Potassium Salt manufacturer ensures the necessary sensitivity and signal-to-noise ratio.

What to Look for When Purchasing:

When you are ready to purchase D-Luciferin Potassium Salt, several factors should guide your choice:

  • Purity: Aim for products with high purity (e.g., ≥98% or ≥99%) to ensure assay sensitivity and reproducibility. Impurities can lead to increased background luminescence or reduced signal intensity.
  • Solubility and Stability: The potassium salt form offers good water solubility, simplifying preparation of working solutions. Proper storage (typically at -20°C, protected from light) is crucial for maintaining product stability. Always check the supplier's recommended storage conditions.
  • Supplier Reputation: Partner with experienced manufacturers and suppliers. Companies with a strong track record in providing high-quality biochemicals and intermediates are more likely to offer reliable products and excellent customer support. If you need to buy D-Luciferin Potassium Salt in bulk, inquire about manufacturer certifications and quality control processes.
  • Price and Lead Time: For bulk purchases, obtaining quotes from multiple suppliers is advisable. Consider the total cost, including shipping and any import duties, as well as the supplier's lead time to ensure your research timeline is met. Exploring options for D-Luciferin Potassium Salt price from Chinese manufacturers can offer significant cost advantages.

Finding a Reliable Supplier:

As a global buyer, identifying a trusted D-Luciferin Potassium Salt supplier is crucial. Many companies specialize in fine chemicals and biochemicals, with a significant presence of manufacturers in China offering competitive pricing and quality products. When you search for a CAS 115144-35-9 manufacturer, look for detailed product specifications, Certificates of Analysis (CoA), and customer testimonials. Engaging with suppliers for a sample or a quotation can be the first step towards establishing a productive partnership.

In conclusion, D-Luciferin Potassium Salt is a foundational reagent for numerous bioluminescence applications. By understanding its properties and prioritizing quality and reliability in your sourcing, you can ensure the success of your critical research projects. When you need to order D-Luciferin Potassium Salt online, remember to prioritize purity and a reputable supply chain.