Mastering Nucleic Acid Staining with Acridine Orange for Research
For any researcher delving into molecular biology or cell diagnostics, the precise identification and visualization of nucleic acids are paramount. Acridine Orange, with its CAS number 10127-02-3, stands out as a remarkably versatile fluorescent dye, offering distinct advantages in differentiating between double-stranded DNA (dsDNA) and single-stranded RNA (ssRNA) or DNA. As a leading manufacturer and supplier based in China, we understand the critical need for high-quality reagents. Acquiring Acridine Orange from a reliable source ensures the accuracy and reproducibility of your experiments.
The core utility of Acridine Orange lies in its differential fluorescence. When it intercalates into the planar structure of dsDNA, it emits a vibrant green fluorescence. Conversely, when it binds electrostatically to the more flexible ssRNA or ssDNA, it exhibits a red fluorescence. This dual-channel capability makes it an indispensable tool for various analyses, from assessing cell viability to quantifying DNA and RNA content within cells. For researchers looking to buy this essential dye, understanding its properties is key to selecting the right grade and supplier.
Beyond its application in nucleic acid staining, Acridine Orange is widely employed in cell cycle studies. Its ability to bind to DNA allows it to stain cells based on their DNA content, enabling the analysis of cell cycle phases and aiding in the study of cell proliferation and apoptosis. Furthermore, its weakly basic nature allows it to accumulate in acidic cellular compartments, such as lysosomes, making it a valuable lysosomal dye for investigating cellular pH and organelle function. The potential to observe these cellular dynamics clearly underscores the importance of sourcing this reagent from a reputable manufacturer.
When considering where to purchase Acridine Orange, it's vital to partner with a company that prioritizes quality and offers competitive pricing. Our commitment as a China-based supplier ensures that you receive a product that meets rigorous standards for purity and performance. We aim to provide researchers with the tools they need to advance their scientific endeavors, whether that’s through detailed DNA/RNA analysis or complex cell cycle investigations. Don't hesitate to request a quote or sample to experience the quality of our Acridine Orange for your laboratory needs.
Perspectives & Insights
Alpha Spark Labs
“For any researcher delving into molecular biology or cell diagnostics, the precise identification and visualization of nucleic acids are paramount.”
Future Pioneer 88
“Acridine Orange, with its CAS number 10127-02-3, stands out as a remarkably versatile fluorescent dye, offering distinct advantages in differentiating between double-stranded DNA (dsDNA) and single-stranded RNA (ssRNA) or DNA.”
Core Explorer Pro
“As a leading manufacturer and supplier based in China, we understand the critical need for high-quality reagents.”