MOPS as a Biological Buffer: Applications in DNA/RNA Extraction
The isolation of high-quality DNA and RNA is a foundational step in countless molecular biology and diagnostic procedures. Success in these processes hinges on maintaining precise environmental conditions, with pH control being particularly critical. 3-Morpholinopropanesulfonic Acid (MOPS, CAS 1132-61-2) has emerged as a premier biological buffer, highly valued for its performance in DNA/RNA extraction protocols. For researchers and diagnostic kit manufacturers, understanding the capabilities of MOPS and finding a reliable source is essential.
MOPS is a zwitterionic buffer belonging to the class of Good's buffers, renowned for their stability and low UV absorbance. Its pKa of 7.20 at 25°C makes it an excellent choice for applications requiring pH maintenance in the physiological range of 6.5 to 7.9. This broad buffering capacity is crucial during the complex steps of DNA/RNA extraction, where varying reagents and conditions are employed.
In DNA/RNA extraction, the initial steps often involve cell lysis, which requires careful pH management to ensure the effective release of nucleic acids while minimizing the degradation of enzymes or cellular components. MOPS buffer provides a stable environment, protecting both the target nucleic acids and the integrity of the extraction process. Its chemical inertness and lack of interaction with biological molecules are key advantages. Many research labs and companies seeking to buy 3-Morpholinopropanesulfonic Acid do so specifically for their DNA/RNA extraction kits.
The benefits of using MOPS extend to protecting nucleic acids from acid hydrolysis or alkaline degradation. By keeping the pH consistently within its effective range, MOPS helps preserve the structural integrity of DNA and RNA, which is vital for downstream analytical techniques like PCR, qPCR, sequencing, and microarrays. When procuring MOPS for these sensitive applications, seeking a high-purity grade (typically 99% min assay) from a trusted 3-Morpholinopropanesulfonic Acid manufacturer is paramount.
Companies that develop and produce DNA/RNA extraction kits rely on consistent and high-quality raw materials. The availability of MOPS in a white crystalline powder form, with strict adherence to specifications, is crucial for reproducible kit performance. Procurement specialists often look for suppliers who can guarantee this quality and provide competitive pricing, especially when searching for a 'price of MOPS buffer for DNA extraction'. A reliable 3-Morpholinopropanesulfonic Acid supplier in China can meet these demands, offering both quality and cost-effectiveness.
Choosing the right buffer is a critical decision in molecular diagnostics and research. MOPS, with its favorable buffering characteristics and purity, is an indispensable tool for successful DNA/RNA extraction. We are dedicated to supplying high-quality MOPS (CAS 1132-61-2) to support the advancement of life sciences. If your laboratory or company requires this essential buffer for your DNA/RNA extraction protocols, we invite you to contact us to learn more about our products and secure a dependable supply chain.
Perspectives & Insights
Logic Thinker AI
“The isolation of high-quality DNA and RNA is a foundational step in countless molecular biology and diagnostic procedures.”
Molecule Spark 2025
“Success in these processes hinges on maintaining precise environmental conditions, with pH control being particularly critical.”
Alpha Pioneer 01
“3-Morpholinopropanesulfonic Acid (MOPS, CAS 1132-61-2) has emerged as a premier biological buffer, highly valued for its performance in DNA/RNA extraction protocols.”