In the precise world of analytical chemistry, the quality of solvents is often the differentiating factor between reliable data and inconclusive results. N-Hexane (CAS 110-54-3), particularly in its high-purity grades, is a cornerstone solvent for chromatographic techniques such as Gas Chromatography (GC) and High-Performance Liquid Chromatography (HPLC). Its specific properties make it indispensable, but also necessitate careful consideration when purchasing to ensure it meets the stringent demands of laboratory analysis.

Why N-Hexane is Essential for Chromatography:

N-Hexane's utility in chromatography stems from its characteristics as a non-polar, volatile organic solvent. In GC, it can serve as a mobile phase, or more commonly, as a solvent for sample preparation and injection. Its low boiling point allows for rapid evaporation after separation, minimizing residual solvent effects. In HPLC, especially in reversed-phase chromatography, n-hexane is frequently used as a component of the mobile phase, often mixed with more polar solvents like acetonitrile or methanol. It is particularly effective for separating non-polar compounds, and its low UV cutoff wavelength makes it suitable for UV detection.

The effectiveness of n-hexane in chromatography is directly tied to its purity. Impurities, even at trace levels, can:

  • Cause baseline noise: Interfering signals that obscure analyte peaks.
  • Lead to ghost peaks: False peaks appearing in the chromatogram, mimicking actual analytes.
  • Affect retention times: Altering the time it takes for compounds to elute, impacting identification.
  • Reduce detector sensitivity: Masking weak analyte signals.

Therefore, when procuring n-hexane for chromatographic applications, it is vital to look for specific grades like HPLC grade, GC grade, or spectroscopic grade, which are manufactured to rigorous purity standards, often exceeding 99.5% or even 99.9%. These grades are typically tested for UV absorbance, residue after evaporation, and trace contaminants.

Sourcing High-Purity N-Hexane: What Buyers Need to Know

For research scientists, laboratory managers, and procurement specialists, selecting the right supplier for analytical-grade n-hexane is critical. When you decide to buy n-hexane for chromatography, consider these points:

  • Supplier Reputation: Partner with established chemical manufacturers and distributors known for their quality control and product consistency.
  • Grade Specification: Clearly define and request the specific grade of n-hexane required for your analytical instruments and methods.
  • Certificates of Analysis (CoA): Always demand a CoA with each batch, detailing the purity and specifications met.
  • Packaging: Ensure appropriate packaging (e.g., amber glass bottles to protect from light, tightly sealed to prevent contamination) is used.
  • Pricing and Availability: While purity is key, competitive pricing and reliable availability are also important factors for sustained laboratory operations.

As a dedicated manufacturer and supplier of industrial and analytical chemicals from China, we offer high-purity n-hexane suitable for all your chromatographic needs. Our commitment to quality assurance ensures that our products deliver the precision and reliability your laboratory demands. We understand the critical nature of these applications and are equipped to provide the necessary product specifications and documentation. Contact us today to source your premium n-hexane and ensure the integrity of your analytical results.