The precise labeling of molecules containing amine functional groups is a cornerstone technique in analytical chemistry, biochemistry, and pharmaceutical research. 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl), CAS 10199-89-0, is a widely utilized reagent for this purpose, renowned for its ability to impart fluorescence upon reaction with amines. As a dedicated supplier of fine chemicals, we highlight the importance of NBD-Cl in amine labeling and guide you on selecting the optimal source.

NBD-Cl's utility stems from its reactivity with primary and secondary amines. Upon reaction, it forms a stable, fluorescent adduct, enabling the detection and quantification of even trace amounts of amine-containing compounds. This makes it an invaluable tool for a variety of applications:

  • Analytical Detection: In chromatography, particularly HPLC, NBD-Cl serves as a derivatizing agent to make poorly detectable analytes visible via fluorescence detection. This is critical for analyzing amino acids, peptides, and amine metabolites in complex biological samples. When you need to buy NBD-Cl for sensitive analyses, ensuring high purity from your supplier is key.
  • Biological Assays: NBD-Cl can be used to label biomolecules like proteins and peptides. This labeling can aid in studying their interactions, tracking their distribution within cells or tissues, or as a component in diagnostic assays.
  • Chemical Synthesis: In synthetic chemistry, NBD-Cl is employed to introduce fluorescent tags onto amine-containing intermediates or final products, facilitating characterization, purification, and the development of novel fluorescent materials.
  • Quality Control: For manufacturers in the pharmaceutical and chemical industries, NBD-Cl can be a valuable reagent in quality control procedures to verify the presence or quantity of amine-containing compounds.

The selection of a reliable supplier for NBD-Cl is paramount to ensure the success of your amine labeling endeavors. Factors such as product purity, consistency, and availability significantly impact experimental outcomes. As a manufacturer located in China, we offer 4-Chloro-7-nitrobenzo-2,1,3-benzadiazole with guaranteed high purity (typically ≥98% GC), ensuring predictable and sensitive labeling reactions.

When considering the price of 4-Chloro-7-nitrobenzo-2,1,3-benzadiazole, it's important to look beyond the per-gram cost. A reputable manufacturer can provide competitive pricing while maintaining stringent quality control, which translates to greater reliability and fewer experimental issues. Direct sourcing from China can offer economic advantages without compromising on the quality required for demanding applications.

In conclusion, NBD-Cl is a powerful fluorescent labeling reagent for a wide array of applications involving amines. Whether for advanced analytical techniques, intricate biological research, or precise chemical synthesis, the quality of your NBD-Cl directly influences your results. We encourage you to buy NBD-Cl from a trusted manufacturer that prioritizes purity and customer satisfaction, ensuring your projects benefit from the dependable performance of this essential fluorescent probe.