Optimizing HRP Assays: The Critical Role of Sodium 3-(10H-phenothiazin-10-yl)propane-1-sulfonate
In the dynamic field of biochemical analysis and diagnostics, the sensitivity and reliability of assays are paramount. Horseradish Peroxidase (HRP)-based detection systems, known for their efficiency and adaptability, are cornerstones of modern laboratory techniques. Central to maximizing the performance of these systems is the careful selection of ancillary reagents, particularly chemiluminescence enhancers. Sodium 3-(10H-phenothiazin-10-yl)propane-1-sulfonate (CAS 101199-38-6), often referred to by its research code PTZ-343, stands out as a highly effective enhancer that significantly elevates signal output. As a dedicated manufacturer and supplier in China, we are committed to providing this critical component to researchers worldwide.
The core function of PTZ-343 within an HRP-luminol chemiluminescent system is to act as a potent enhancer. It facilitates the electron transfer process, stabilizing the key intermediates that lead to light emission. This stabilization results in a marked increase in the intensity and duration of the luminescent signal, which is crucial for detecting low-abundance targets. For procurement managers and researchers seeking to buy reagents that improve assay sensitivity, PTZ-343 is an excellent choice. Its application ensures that even trace amounts of analytes can be reliably quantified.
The chemical structure of Sodium 3-(10H-phenothiazin-10-yl)propane-1-sulfonate, featuring a phenothiazine ring system modified with a propane sulfonate moiety, contributes to its superior performance. The sulfonate group enhances water solubility, a practical advantage for its incorporation into aqueous assay buffers. Furthermore, the electron-rich nature of the phenothiazine core allows for efficient interaction with HRP and the luminol reaction intermediates. This molecular design makes it a preferred choice over many other enhancers, especially when high signal-to-noise ratios are required. When sourcing this compound, purity is key, and our product guarantees an assay of no less than 99%.
As a leading supplier from China, we understand the importance of consistent quality and reliable supply chains. Our manufacturing processes for PTZ-343 are optimized for both purity and scalability, ensuring that we can meet the demands of research institutions, diagnostic companies, and contract research organizations. For those looking to purchase this enhancer, we offer competitive pricing and flexible order quantities, making it accessible for various project scales. Investing in a high-quality enhancer like ours can significantly reduce the need for repeat experiments and improve overall research efficiency.
In conclusion, Sodium 3-(10H-phenothiazin-10-yl)propane-1-sulfonate is a vital reagent for anyone aiming to optimize HRP-based chemiluminescence assays. Its ability to amplify signal and improve detection limits makes it a powerful tool for advancing research and diagnostics. We encourage you to contact us to discuss your needs and discover how our high-purity CAS 101199-38-6 can be a valuable asset to your laboratory.
Perspectives & Insights
Molecule Vision 7
“Its application ensures that even trace amounts of analytes can be reliably quantified.”
Alpha Origin 24
“The chemical structure of Sodium 3-(10H-phenothiazin-10-yl)propane-1-sulfonate, featuring a phenothiazine ring system modified with a propane sulfonate moiety, contributes to its superior performance.”
Future Analyst X
“The sulfonate group enhances water solubility, a practical advantage for its incorporation into aqueous assay buffers.”