In the intricate field of cell biology and biotechnology, monitoring the health and growth of cell cultures is fundamental to research success. This often involves using specific indicators that provide visual cues about the culture's environment and the metabolic activity of the cells. Phenol Red, a widely used pH indicator known by its CAS number 143-74-8, plays a significant role in this domain, particularly in assays related to cell viability and proliferation. Its ability to signal changes in medium pH due to cellular metabolism makes it an invaluable tool for researchers. If you are looking to buy Phenol Red for your cell culture needs, NINGBO INNO PHARMCHEM CO.,LTD., a leading supplier in China, offers high-quality solutions.

Phenol Red’s utility in cell culture stems from its well-defined pH transition range. As cells metabolize nutrients and produce waste products, the pH of the culture medium typically decreases. Phenol Red, present in the medium, changes color from its typical red state (indicating a neutral to slightly alkaline pH, suitable for most cell growth) to yellow as the acidity increases. This color shift provides an immediate visual alert that the culture's environment is changing, often indicating a buildup of acidic metabolites or the depletion of essential buffering components. This visual monitoring is critical for maintaining optimal conditions for cell viability and ensuring that cells are actively proliferating as expected.

The application of Phenol Red in cell viability and proliferation studies is multifaceted. Researchers use it to assess the responsiveness of cells to various treatments or growth factors. For instance, if a treatment is expected to enhance cell metabolism, an accompanying decrease in medium pH (indicated by Phenol Red turning yellow) might be observed. Conversely, if a treatment inhibits cell growth, the medium might remain red for longer. This makes Phenol Red a cost-effective and convenient way to qualitatively assess cellular activity without relying solely on more complex assays. The reliability of Phenol Red is paramount, which is why NINGBO INNO PHARMCHEM CO.,LTD. focuses on providing high-purity grades suitable for these sensitive applications.

Furthermore, while Phenol Red is primarily a pH indicator, its presence in cell culture media is generally considered safe and non-inhibitory to most cell lines, provided it is used at recommended concentrations. However, it is important to note that some research protocols, particularly those involving sensitive pH studies or specific cell types, may require Phenol Red-free media. For general cell culture and routine monitoring of cell viability and proliferation, Phenol Red remains a standard component. NINGBO INNO PHARMCHEM CO.,LTD., as a dedicated manufacturer and supplier in China, is committed to supporting the scientific community with essential reagents like Phenol Red (CAS 143-74-8). We invite you to explore our product offerings and experience our commitment to quality and customer satisfaction.