In the realm of scientific research, the efficacy of biochemical experiments hinges on meticulous control over reaction conditions, with pH stability being a paramount factor. Biological buffers are indispensable tools that achieve this stability. Among these, DIPSO (CAS 68399-80-4) is a notable zwitterionic buffer with a distinct set of properties beneficial for specific research applications. As a dedicated manufacturer and supplier of high-purity biochemicals in China, we aim to elucidate the characteristics of DIPSO and guide researchers in its effective utilization.

Chemical Identity and Physical Characteristics

DIPSO, formally known as 3-[N,N-di(hydroxyethyl)amino]-2-hydroxypropanesulfonic acid, is classified as a zwitterionic buffer due to its internal charge separation. It typically presents as a fine white crystalline powder. A key characteristic of DIPSO is its limited solubility in common organic solvents, which can be an important consideration depending on the experimental setup. Conversely, its solubility in aqueous solutions is sufficient for buffering applications, maintaining a consistent performance across a usable pH range.

Buffering Performance and pH Range

DIPSO is highly effective in maintaining a stable pH between 7.0 and 8.2. This pH range is significant as it closely mirrors physiological conditions and is suitable for a variety of enzymatic reactions and protein studies. The zwitterionic nature of DIPSO allows it to effectively buffer against both acidic and basic additions, ensuring that the pH remains within the desired narrow window. This reliability makes it an excellent choice for sensitive biochemical assays where pH drift could invalidate results. Researchers looking to buy DIPSO buffer for such critical applications will find our product offers consistent performance.

Applications in Scientific Research

The specific properties of DIPSO lend themselves well to several key research areas:

1. Protein Separation and Purification: Its buffering capabilities are highly valued in chromatographic techniques aimed at purifying proteins. By maintaining a stable pH, DIPSO aids in the specific binding and elution of proteins, thereby improving the efficiency and purity of the isolated compounds.

2. Electrophoresis: DIPSO serves as a reliable component in electrophoresis buffer systems, contributing to the stable ionic environment necessary for the accurate separation of biomolecules based on their charge and size.

Important Considerations for Use

While DIPSO is a valuable buffer, its tendency to form complexes with certain metal ions, leading to precipitation, is a critical point to note. This characteristic makes it less ideal for cell culture media where the presence of essential metal cations is required. Researchers should factor this into their experimental design. If your work involves metal ions or requires a buffer outside DIPSO's effective pH range, exploring alternative buffers is recommended.

Your Trusted Source for DIPSO Buffer

At our facility, we are committed to producing and supplying DIPSO (CAS 68399-80-4) of the highest purity. We understand that the quality of your reagents directly impacts the reliability of your research. Therefore, we adhere to stringent quality control protocols throughout our manufacturing process. By choosing us as your supplier, you ensure access to consistently high-quality biochemicals, backed by expert technical support. Partner with us to reliably buy DIPSO buffer and elevate your research.