5-Sulfosalicylic Acid Dihydrate (CAS 5965-83-3) is a cornerstone reagent in various biochemical and analytical procedures, most notably in the quantitative determination of protein concentration in biological samples. Its efficacy stems from its ability to precipitate proteins, forming stable complexes that can be easily measured.

The mechanism involves the acidic nature of sulfosalicylic acid. In solution, it dissociates, and the sulfonate group imparts increased water solubility while the carboxyl and hydroxyl groups contribute to its reactivity. When added to a sample containing proteins, it denatures them and forms insoluble complexes. This precipitation is the basis for colorimetric assays, where the turbidity or color intensity of the resulting complex is directly proportional to the protein concentration. This method is widely adopted for its simplicity and reliability, especially in clinical settings for urine protein testing.

Beyond protein assays, this versatile compound functions as a biochemical reagent and an analytical reagent. Its capacity to chelate with various metal ions makes it useful in colorimetric analysis for determining levels of elements like iron, aluminum, and others. This broad applicability makes it a staple in many quality control and research laboratories.

For procurement, it is essential to source 5-Sulfosalicylic Acid Dihydrate from reputable manufacturers and suppliers who guarantee high purity, typically above 99%. Such suppliers ensure that the reagent's performance is consistent and reliable, crucial for accurate scientific data. If you are looking to buy this essential chemical for your protein determination needs or other analytical applications, consider our offerings. As a dedicated supplier, we provide high-quality 5-Sulfosalicylic Acid Dihydrate at competitive prices. Contact us today for a quote and to secure your supply.