Alcohol Oxidase (EC 1.1.3.13), recognized by its CAS number 9073-63-6, is a fascinating flavoenzyme that exemplifies efficient biological catalysis. Understanding its intricate enzymatic function is key to appreciating its utility in various scientific and industrial applications. As a dedicated manufacturer and supplier, we are passionate about sharing the science behind this vital enzyme.

At its core, Alcohol Oxidase catalyzes a pivotal reaction: the oxidation of primary alcohols to their corresponding aldehydes, with the simultaneous production of hydrogen peroxide (H2O2). This reaction, RCH2OH + O2 → RCHO + H2O2, is fundamental to the metabolism of alcohols, particularly methanol, in various microorganisms. The enzyme utilizes flavin adenine dinucleotide (FAD) as a cofactor, which is crucial for electron transfer during the oxidation process.

The enzyme's substrate specificity is a notable characteristic. Alcohol Oxidase demonstrates a high affinity for short-chain, linear primary alcohols, with methanol being the preferred substrate, followed by ethanol and other short-chain alcohols. It is less effective or inactive with branched-chain alcohols, aldehydes, ketones, or organic acids. This specificity is precisely what makes it invaluable when you purchase Alcohol Oxidase for targeted applications like biosensor development or specific chemical synthesis pathways.

Furthermore, Alcohol Oxidase is primarily localized within the peroxisomes of cells, although it can also be found in the cytoplasm. This localization is linked to its role in energy metabolism, especially in yeasts that utilize methanol. For researchers studying cellular respiration, enzyme localization, or metabolic engineering, our high-quality Alcohol Oxidase product offers a reliable tool for investigation. As a trusted supplier, we ensure the enzyme's integrity for such studies.

The relative stability of Alcohol Oxidase, including its resistance to inactivation by hydrogen peroxide and certain metal ions, further enhances its applicability. This robustness allows for its use in a broader range of conditions and storage scenarios, making it a practical choice for industrial processes. When you choose to buy Alcohol Oxidase from us, you are investing in an enzyme whose scientific underpinnings translate directly into practical advantages. Contact us to learn more about the science of Alcohol Oxidase and how our products can support your work.