Bromophenol Blue Sodium Salt (CAS 34725-61-6) is a fascinating compound whose utility stems from fundamental principles of chemistry and molecular biology. Its well-defined chromophoric properties and electrochemical behavior make it an indispensable tool in various laboratory settings. As a leading supplier of high-quality chemical reagents, we are pleased to share insights into the science that underpins its effectiveness, highlighting why researchers worldwide rely on this compound for accurate analysis.

The functionality of Bromophenol Blue Sodium Salt as a pH indicator is rooted in the phenomenon of acid-base equilibrium and structural changes within the molecule. As a sulfonphthalein dye, it undergoes a reversible structural transformation in response to changes in hydrogen ion concentration. In acidic solutions (below pH 3.0), the molecule exists in a form that absorbs light in the UV-visible spectrum in a manner that results in a yellow appearance. As the pH increases towards the transition range (3.0-4.6), protonation and deprotonation events alter the electronic configuration of the molecule, leading to a shift in light absorption and the observed color change to blue. This sensitivity to pH makes it a valuable component in titration experiments, where precise determination of the equivalence point is critical. When seeking to buy such indicators, purity is key to ensuring the accuracy of these critical measurements.

In the realm of molecular biology, Bromophenol Blue Sodium Salt plays a vital role as a tracking dye in gel electrophoresis. This technique separates biological molecules, such as DNA and proteins, based on their size and charge as they migrate through a porous gel matrix under an electric field. Bromophenol Blue Sodium Salt is a negatively charged molecule that migures at a predictable rate through standard electrophoresis gels. Its visible color allows researchers to visually track the movement of the electric front and, by extension, the progress of the biomolecules being separated. This visual tracking is crucial for optimizing run times and preventing the loss of samples by over-electrophoresis. The availability of this dye from reliable manufacturers ensures that labs can consistently procure this essential component for their molecular analyses.

The chemical structure of Bromophenol Blue Sodium Salt, characterized by its bromine substituents and sulfonphthalein core, contributes to its stability and distinct spectral properties. These features are precisely what make it suitable for both qualitative pH observations and quantitative monitoring in electrophoresis. Laboratories and research institutions seeking to purchase this compound can benefit from the expertise of dedicated chemical suppliers who ensure rigorous quality control. By understanding the scientific basis of its function, users can better appreciate the importance of selecting high-purity Bromophenol Blue Sodium Salt for their critical analytical and biological applications.